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Appl Environ Microbiol, July 1998, p. 2716-2720, Vol. 64, No. 7
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Design and Application of Two Rapid Screening Techniques for Isolation of Mn(IV) Reduction-Deficient Mutants of Shewanella putrefaciens

Brian S. Burnes, Michael J. Mulberry, and Thomas J. DiChristina*

School of Biology, Georgia Institute of Technology, Atlanta, Georgia 30332

Received 12 December 1997/Accepted 27 April 1998

Chemical mutagenesis procedures and two newly developed rapid plate assays were used to identify two Mn(IV) reduction-deficient (Mnr) mutants of Shewanella putrefaciens. All eleven members of a set of previously isolated Fe(III) reduction-deficient (Fer) mutants displayed Mnr-positive phenotypes on the plate assays and were also capable of anaerobic growth on Mn(IV) as the sole terminal electron acceptor.

* Corresponding author. Mailing address: Georgia Institute of Technology, School of Biology, Atlanta, GA 30332. Phone: (404) 894-8419. Fax: (404) 894-0519. E-mail: thomas.dichristina{at}biology.gatech.edu.

Appl Environ Microbiol, July 1998, p. 2716-2720, Vol. 64, No. 7
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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