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Appl Environ Microbiol, July 1998, p. 2721-2722, Vol. 64, No. 7
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Use of Luciferase Genes as Biosensors To Study Bacterial Physiology in the Digestive Tract

G. Corthier,1,* C. Delorme,2 S. D. Ehrlich,2 and P. Renault2

Unite d'Ecologie et de Physiologie Digestive-FBI1 and Génétique Microbienne,2 Institut National de la Recherche Agronomique, 78352 Jouy-en-Josas Cedex, France

Received 25 November 1997/Accepted 15 April 1998

A method based on the use of the bacterial luciferase genes was developed in order to investigate Lactococcus lactis gene expression in the mouse digestive tract. Germfree mice were monoassociated with different strains containing transcriptional fusions of promoters with the luciferase genes. Our results demonstrate that this method is readily applicable to the study of promoter strength and physiology of bacteria in the digestive tract.

* Corresponding author. Mailing address: Unite d'Ecologie et de Physiologie Digestive, Unite FBI, Centre de Recherches de Jouy, INRA, 78352 Jouy-en-Josas Cedex, France. Phone: 01 34 65 24 67. Fax: 01 34 65 24 62. E-mail: corthier{at}biotec.jouy.inra.fr.

Appl Environ Microbiol, July 1998, p. 2721-2722, Vol. 64, No. 7
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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