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Appl Environ Microbiol, July 1998, p. 2755-2759, Vol. 64, No. 7
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Microbial Characterization of Biological Filters Used for Drinking Water Treatment

Deborah M. Moll,1 R. Scott Summers,1,* and Alec Breen2,dagger

Department of Civil and Environmental Engineering1 and Department of Molecular Genetics,2 University of Cincinnati, Cincinnati, Ohio 45221

Received 22 December 1997/Accepted 7 April 1998

The impact of preozonation and filter contact time (depth) on microbial communities was examined in drinking water biofilters treating Ohio River water which had undergone conventional treatment (coagulation, flocculation, sedimentation) or solutions of natural organic matter isolated from groundwater (both ozonated and nonozonated). With respect to filter depth, compared to filters treating nonozonated waters, preozonation of treated water led to greater differences in community phospholipid fatty acid (PLFA) profiles, utilization of sole carbon sources (Biolog), and arbitrarily primed PCR fingerprints. PLFA profiles indicated that there was a shift toward anaerobic bacteria in the communities found in the filter treating ozonated water compared to the communities found in the filter treating nonozonated settled water, which had a greater abundance of eukaryotic markers.

* Corresponding author. Mailing address: Department of Civil and Environmental Engineering, University of Cincinnati, P.O. Box 210071, Cincinnati, OH 45221-0071. Phone: (513) 556-3692. Fax: (513) 556-2599. E-mail: rsummers{at}boss.cee.uc.edu.

dagger Present address: Betz Dearborn, Inc., Jacksonville, FL 32256.

Appl Environ Microbiol, July 1998, p. 2755-2759, Vol. 64, No. 7
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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