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Applied and Environmental Microbiology, August 1998, p. 3092-3095, Vol. 64, No. 8
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The Requirement of RpoN (Sigma Factor sigma 54) in Denitrification by Pseudomonas stutzeri Is Indirect and Restricted to the Reduction of Nitrite and Nitric Oxide

Elisabeth Härtig and Walter G. Zumft*

Lehrstuhl für Mikrobiologie der Universität Karlsruhe, D-76128 Karlsruhe, Germany

Received 15 January 1998/Accepted 24 May 1998

The rpoN region of Pseudomonas stutzeri was cloned, and an rpoN null mutant was constructed. RpoN was not essential for denitrification in this bacterium but affected the expression levels and enzymatic activities of cytochrome cd1 nitrite reductase and nitric oxide reductase, whereas those of respiratory nitrate reductase and nitrous oxide reductase were comparable to wild-type levels. Since the transcription of the structural genes nirS and norCB, coding for nitrite reductase and the nitric oxide reductase complex, respectively, proceeded unabated, our data indicate a posttranslational process for the two key enzymes of denitrification depending on RpoN.

* Corresponding author. Mailing address: Lehrstuhl für Mikrobiologie, Universität Karlsruhe, P.O. Box 6980, D-76128 Karlsruhe, Germany. Phone: 49 (721) 608 3474. Fax: 49 (721) 608 4920. E-mail: dj03{at}rz.uni-karlsruhe.de.

Applied and Environmental Microbiology, August 1998, p. 3092-3095, Vol. 64, No. 8
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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