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Applied and Environmental Microbiology, August 1998, p. 3096-3098, Vol. 64, No. 8
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Differentiation of Dextran-Producing Leuconostoc Strains by a Modified Randomly Amplified Polymorphic DNA Protocol

Scott M. Holt1,* and Gregory L. Cote2

Department of Biological Sciences, Western Illinois University, Macomb,1 and Biopolymer Research Unit, National Center for Agricultural Utilization Research, USDA Agricultural Research Service, Peoria,2 Illinois

Received 26 January 1998/Accepted 1 June 1998

Seven dextran-producing Leuconostoc strains were differentiated by using a modified randomly amplified polymorphic DNA (RAPD) protocol that incorporated specific primers designed from conserved regions of dextransucrase genes. RAPD profiles showed intraspecies differences among the Leuconostoc mesenteroides strains tested. This modified RAPD protocol will aid in the differentiation of polymer-producing leuconostocs, which are currently distinguished by time-consuming analyses of the dextrans they synthesize.

* Corresponding author. Mailing address: Western Illinois University, Department of Biological Sciences, 1 University Circle, Waggoner Hall 316, Macomb, IL 61455. Phone: (309) 298-1484. Fax: (309) 298-2270. E-mail: smholt{at}ccmail.wiu.edu.

Applied and Environmental Microbiology, August 1998, p. 3096-3098, Vol. 64, No. 8
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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