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Applied and Environmental Microbiology, September 1998, p. 3264-3269, Vol. 64, No. 9
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Biosensor Determination of the Microscale Distribution of Nitrate, Nitrate Assimilation, Nitrification, and Denitrification in a Diatom-Inhabited Freshwater Sediment

Jan Lorenzen, Lars Hauer Larsen, Thomas Kjær, and Niels-Peter Revsbech*

Department of Microbial Ecology, University of Aarhus, Ny Munkegade, DK-8000 Aarhus C, Denmark

Received 26 February 1998/Accepted 17 June 1998

High-resolution NO3- profiles in freshwater sediment covered with benthic diatoms were obtained with a new microscale NO3- biosensor characterized by absence of interference from chemical species other than NO2- and N2O. Analysis of the microprofiles obtained indicated no nitrification during darkness, high rates of nitrification and a tight coupling between nitrification and denitrification during illumination, and substantial rates of NO3- assimilation during illumination. Nitrification during darkness could be induced by purging the bulk water with O2 gas, indicating that the stimulatory effect on nitrification by illumination was caused by algal production of O2. NH4+ addition did not stimulate nitrification during darkness when O2 was restricted to the upper 1-mm layer, and there was thus a low nitrification potential in the permanently oxic top 1 mm of the sediment.

* Corresponding author. Mailing address: Department of Microbial Ecology, University of Aarhus, Ny Munkegade, Building 540, DK-8000, Aarhus C, Denmark. Phone: 45 89 42 32 44. Fax: 45 86 12 71 91. E-mail: revsbech{at}biology.aau.dk.

Applied and Environmental Microbiology, September 1998, p. 3264-3269, Vol. 64, No. 9
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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