This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Björkroth, K. J.
Right arrow Articles by Korkeala, H. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Björkroth, K. J.
Right arrow Articles by Korkeala, H. J.
Agricola
Right arrow Articles by Björkroth, K. J.
Right arrow Articles by Korkeala, H. J.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, September 1998, p. 3313-3319, Vol. 64, No. 9
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Identification and Characterization of Leuconostoc carnosum, Associated with Production and Spoilage of Vacuum-Packaged, Sliced, Cooked Ham

K. J. Björkroth,1,* P. Vandamme,2 and H. J. Korkeala1

Department of Food and Environmental Hygiene, University of Helsinki, Helsinki, Finland,1 and Department of Microbiology, University of Ghent, Ghent, Belgium2

Received 26 February 1998/Accepted 15 June 1998

Leuconostoc carnosum was shown to be the specific spoilage organism in vacuum-packaged, sliced, cooked ham showing spoilage during 3 weeks of shelf life. Identification of the specific spoilage organism was done by use of phenotypic data and ClaI, EcoRI, and HindIII reference strain ribopatterns. One hundred L. carnosum isolates associated with the production and spoilage of the ham were further characterized by pulsed-field gel electrophoresis (PFGE), together with some meat-associated Leuconostoc species: L. citreum, L. gelidum, L. mesenteroides subsp. dextranicum, and L. mesenteroides subsp. mesenteroides. ApaI and SmaI digests divided the industrial L. carnosum strains into 25 different PFGE types, ApaI and SmaI types being consistent. Only one specific PFGE type was associated with the spoiled packages. This type also was detected in air and raw-meat mass samples. The spoilage strain did not produce bacteriocins. Only seven isolates belonging to three different PFGE types produced bacteriocins. Similarity analysis of the industrial L. carnosum strains revealed a homogeneous cluster which could be divided into eight subclusters consisting of strains having at most three-fragment differences. The L. carnosum cluster was clearly distinguished from the other meat-associated leuconostoc clusters, with the exception of the L. carnosum type strain. Ribotyping can be very helpful in the identification of L. carnosum, but its discriminatory power is too weak for strain characterization. PFGE provides good discrimination for studies dealing with the properties of homogeneous L. carnosum strains.

* Corresponding author. Mailing address: Department of Food and Environmental Hygiene, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 57, FIN-00014 Helsinki, Finland. Phone: 358-50-5976555. Fax: 358-9-70849718. E-mail: johanna.björkroth{at}helsinki.fi.

Applied and Environmental Microbiology, September 1998, p. 3313-3319, Vol. 64, No. 9
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Tominaga, T., Hatakeyama, Y. (2007). Development of Innovative Pediocin PA-1 by DNA Shuffling among Class IIa Bacteriocins. Appl. Environ. Microbiol. 73: 5292-5299 [Abstract] [Full Text]  
  • Vihavainen, E., Lundstrom, H.-S., Susiluoto, T., Koort, J., Paulin, L., Auvinen, P., Bjorkroth, K. J. (2007). Role of Broiler Carcasses and Processing Plant Air in Contamination of Modified-Atmosphere-Packaged Broiler Products with Psychrotrophic Lactic Acid Bacteria. Appl. Environ. Microbiol. 73: 1136-1145 [Abstract] [Full Text]  
  • Svec, P., Vancanneyt, M., Koort, J., Naser, S. M., Hoste, B., Vihavainen, E., Vandamme, P., Swings, J., Bjorkroth, J. (2005). Enterococcus devriesei sp. nov., associated with animal sources. Int. J. Syst. Evol. Microbiol. 55: 2479-2484 [Abstract] [Full Text]  
  • Koort, J., Murros, A., Coenye, T., Eerola, S., Vandamme, P., Sukura, A., Bjorkroth, J. (2005). Lactobacillus oligofermentans sp. nov., Associated with Spoilage of Modified-Atmosphere-Packaged Poultry Products. Appl. Environ. Microbiol. 71: 4400-4406 [Abstract] [Full Text]  
  • Helmark, S., Hansen, M. E., Jelle, B., Sorensen, K. I., Jensen, P. R. (2004). Transformation of Leuconostoc carnosum 4010 and Evidence for Natural Competence of the Organism. Appl. Environ. Microbiol. 70: 3695-3699 [Abstract] [Full Text]  
  • Hamasaki, Y., Ayaki, M., Fuchu, H., Sugiyama, M., Morita, H. (2003). Behavior of Psychrotrophic Lactic Acid Bacteria Isolated from Spoiling Cooked Meat Products. Appl. Environ. Microbiol. 69: 3668-3671 [Abstract] [Full Text]  
  • Rudi, K., Flateland, S. L., Hanssen, J. F., Bengtsson, G., Nissen, H. (2002). Development and Evaluation of a 16S Ribosomal DNA Array-Based Approach for Describing Complex Microbial Communities in Ready-To-Eat Vegetable Salads Packed in a Modified Atmosphere. Appl. Environ. Microbiol. 68: 1146-1156 [Abstract] [Full Text]  
  • Björkroth, K. J., Geisen, R., Schillinger, U., Weiss, N., De Vos, P., Holzapfel, W. H., Korkeala, H. J., Vandamme, P. (2000). Characterization of Leuconostoc gasicomitatum sp. nov., Associated with Spoiled Raw Tomato-Marinated Broiler Meat Strips Packaged under Modified-Atmosphere Conditions. Appl. Environ. Microbiol. 66: 3764-3772 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»