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Applied and Environmental Microbiology, January 1999, p. 287-290, Vol. 65, No. 1
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Rapid Detection of Ophiostoma piceae and O. quercus in Stained Wood by PCR

Seong Hwan Kim, Adnan Uzunovic, and Colette Breuil*

Chair of Forest Products Biotechnology, Department of Wood Science, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4

Received 6 August 1998/Accepted 3 November 1998

A rapid, sensitive, and simple method was developed to detect the sapstain fungi Ophiostoma piceae and O. quercus in stained wood. By using microwave heating for DNA extraction and PCR with internal transcribed spacer-derived-specific primers, detection was feasible within 4 h, even with DNA obtained from a single synnema. This method can easily be extended for the detection of other wood-inhabiting fungi.

* Corresponding author. Mailing address: Chair of Forest Products Biotechnology, Department of Wood Science, University of British Columbia, Vancouver, BC, Canada V6T 1Z4. Phone: 1-604-822-9738. Fax: 1-604-822-9104. E-mail: breuil{at}interchg.ubc.ca.

Applied and Environmental Microbiology, January 1999, p. 287-290, Vol. 65, No. 1
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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  • Alamouti, S. M., Kim, J.-J., Breuil, C. (2006). A new Leptographium species associated with the northern spruce engraver, Ips perturbatus, in western Canada.. Mycologia 98: 149-160 [Abstract] [Full Text]  


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