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Applied and Environmental Microbiology, January 1999, p. 301-306, Vol. 65, No. 1
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Conditions for Vigorous Growth on Sulfide and Reactor-Scale Cultivation Protocols for the Thermophilic Green Sulfur Bacterium Chlorobium tepidum

Biswarup Mukhopadhyay,* Eric F. Johnson, and Manuel Ascano Jr.dagger

Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801

Received 14 August 1998/Accepted 4 November 1998

We describe a reactor-scale cultivation protocol for the fastest-growing and only known thermophilic member of the family Chlorobiaceae, Chlorobium tepidum. We discovered that C. tepidum would grow with sulfide as the sole electron source at rates and with final cell yields comparable to those found with thiosulfate only if the sulfide concentration was maintained below 0.1 mM and the culture redox potential was at -300 ± 20 mV. Such was also the requirement for growth in a photobioreactor when thiosulfate (optimum level, 12 mM) was used as the preferred electron source. For cultivation of C. tepidum on a 5- to 500-ml scale, we used the system of Balch and Wolfe (Appl. Environ. Microbiol. 32:781-791, 1976) using stopper-sealed serum tubes and bottles as an alternative to the methods commonly used for the cultivation of phototrophic anaerobes and obtained consistent results.

* Corresponding author. Mailing address: University of Illinois at Urbana-Champaign, Department of Microbiology, B103 Chemical and Life Sciences Laboratory, 601 S. Goodwin Ave., Urbana, IL 61801. Phone: (217) 333-1397. Fax: (217) 244-8485. E-mail: biswarup{at}pop.life.uiuc.edu.

dagger Present address: Proctor & Gamble Pharmaceuticals, Health Care Research Center, Mason, OH 45040.

Applied and Environmental Microbiology, January 1999, p. 301-306, Vol. 65, No. 1
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Chan, L.-K., Morgan-Kiss, R. M., Hanson, T. E. (2009). Functional Analysis of Three Sulfide:Quinone Oxidoreductase Homologs in Chlorobaculum tepidum. J. Bacteriol. 191: 1026-1034 [Abstract] [Full Text]  
  • Chan, L.-K., Weber, T. S., Morgan-Kiss, R. M., Hanson, T. E. (2008). A genomic region required for phototrophic thiosulfate oxidation in the green sulfur bacterium Chlorobium tepidum (syn. Chlorobaculum tepidum). Microbiology 154: 818-829 [Abstract] [Full Text]  
  • Hanson, T. E., Tabita, F. R. (2001). A ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO)-like protein from Chlorobium tepidum that is involved with sulfur metabolism and the response to oxidative stress. Proc. Natl. Acad. Sci. USA 10.1073/pnas.081610398v1 [Abstract] [Full Text]  
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