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Applied and Environmental Microbiology, January 1999, p. 351-354, Vol. 65, No. 1
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Persistence of Colonization of Human Colonic Mucosa by a Probiotic Strain, Lactobacillus rhamnosus GG, after Oral Consumption

Minna Alander,1 Reetta Satokari,1 Riitta Korpela,2 Maija Saxelin,2 Terttu Vilpponen-Salmela,3 Tiina Mattila-Sandholm,1 and Atte von Wright1,*

VTT Biotechnology and Food Research, FIN-02044 VTT,1 Valio Ltd. Research and Development Centre, FIN-00101 Helsinki,2 and Harjula Hospital, FIN-70101 Kuopio,3 Finland

Received 8 June 1998/Accepted 30 September 1998

Lactobacillus rhamnosus GG is one of the most thoroughly studied probiotic strains. Its advantages in the treatment of gastrointestinal disorders are well documented. The aim of the present study was to demonstrate with colonic biopsies the attachment of strain GG to human intestinal mucosae and the persistence of the attachment after discontinuation of GG administration. A whey drink fermented with strain GG was fed to human volunteers for 12 days. Fecal samples were collected before, during, and after consumption. L. rhamnosus GG-like colonies were detected in both fecal and colonic biopsy samples. Strain GG was identified by its characteristic colony morphology, a lactose fermentation test, and PCR. This study showed that strain GG was able to attach in vivo to colonic mucosae and, although the attachment was temporary, to remain for more than a week after discontinuation of GG administration. The results demonstrate that the study of fecal samples alone is not sufficient in evaluating colonization by a probiotic strain.

* Corresponding author. Present address: University of Kuopio, Department of Biochemistry and Biotechnology, P.O. Box 1627, FIN-70211 Kuopio, Finland. Phone: 358 17 162087. Fax: 358 17 2811510. E-mail: Atte.vonWright{at}uku.fi.

Applied and Environmental Microbiology, January 1999, p. 351-354, Vol. 65, No. 1
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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