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Applied and Environmental Microbiology, October 1999, p. 4659-4665, Vol. 65, No. 10
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Unexpected Population Distribution in a Microbial Mat Community: Sulfate-Reducing Bacteria Localized to the Highly Oxic Chemocline in Contrast to a Eukaryotic Preference for Anoxia

Dror Minz,1,dagger Susan Fishbain,1 Stefan J. Green,1 Gerard Muyzer,2,Dagger Yehuda Cohen,3 Bruce E. Rittmann,1 and David A. Stahl1,*

Department of Civil Engineering, Northwestern University, Evanston, Illinois 60208-31091; Max-Planck-Institute for Marine Microbiology, D-28359 Bremen, Germany2; and The Moshe Shilo Center for Marine Biogeochemistry, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel3

Received 16 April 1999/Accepted 20 July 1999

The distribution and abundance of sulfate-reducing bacteria (SRB) and eukaryotes within the upper 4 mm of a hypersaline cyanobacterial mat community were characterized at high resolution with group-specific hybridization probes to quantify 16S rRNA extracted from 100-µm depth intervals. This revealed a preferential localization of SRB within the region defined by the oxygen chemocline. Among the different groups of SRB quantified, including members of the provisional families "Desulfovibrionaceae" and "Desulfobacteriaceae," Desulfonema-like populations dominated and accounted for up to 30% of total rRNA extracted from certain depth intervals of the chemocline. These data suggest that recognized genera of SRB are not necessarily restricted by high levels of oxygen in this mat community and the possibility of significant sulfur cycling within the chemocline. In marked contrast, eukaryotic populations in this community demonstrated a preference for regions of anoxia.

* Corresponding author. Mailing address: Department of Civil Engineering, Northwestern University, Evanston, IL 60208-3109. Phone: (847) 491-4997. Fax: (847) 491-4011. E-mail: d-stahl{at}nwu.edu.

dagger Present address: Soil Microbiology, Volcani Research Center, Bet-Dagan, Israel.

Dagger Present address: Netherlands Institute for Sea Research, Den Burg, The Netherlands.

Applied and Environmental Microbiology, October 1999, p. 4659-4665, Vol. 65, No. 10
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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