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Applied and Environmental Microbiology, October 1999, p. 4677-4681, Vol. 65, No. 10
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Viability and DNA Maintenance in Nonculturable Spiral Campylobacter jejuni Cells after Long-Term Exposure to Low Temperatures

Beatriz Lázaro,1 Jose Cárcamo,2 Ana Audícana,2 Ildefonso Perales,2 and Aurora Fernández-Astorga1,*

Departamento de Inmunología, Microbiología y Parasitología, Facultad de Farmacia, Universidad del País Vasco (UPV/EHU), 01080 Vitoria-Gasteiz,1 and Laboratorio Normativo de Salud Pública, Departamento de Sanidad, Gobierno Vasco (GV/EJ), 48010 Bilbao,2 Spain

Received 7 June 1999/Accepted 10 July 1999

Survival of Campylobacter jejuni at 4 and 20°C was investigated by using cellular integrity, respiratory activity, two-dimensional (2D) protein profile, and intact DNA content as indicators of potential viability of nonculturable cells. Intact DNA content after 116 days, along with cellular integrity and respiring cells, was detected for up to 7 months at 4°C by pulsed-field gel electrophoresis. Most changes in 2D protein profiles involved up- or down-regulation.

* Corresponding author. Mailing address: Departamento de Inmunologia, Microbiologia y Parasitologia, Facultad de Farmacia, Universidad del Pais Vasco (UPV/EHU), Paseo de la Universidad, 7, 01006 Vitoria-Gasteiz, Spain. Phone: 34 945 013909. Fax: 34 945 130756. E-mail: oipfeasa{at}vf.ehu.es.

Applied and Environmental Microbiology, October 1999, p. 4677-4681, Vol. 65, No. 10
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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