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Applied and Environmental Microbiology, November 1999, p. 5139-5141, Vol. 65, No. 11
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Gene Transfer in the Gastrointestinal Tract

Trudy Netherwood,1,2,* R. Bowden,1,2 P. Harrison,1,2 A. G. O'Donnell,2 D. S. Parker,1 and H. J. Gilbert1

Department of Biological and Nutritional Sciences1 and Department of Agriculture and Environmental Science,2 University of Newcastle-upon-Tyne, Newcastle-upon-Tyne NE1 7RU, Great Britain

Received 26 April 1999/Accepted 9 August 1999

The maximum in vivo transfer rate of plasmid pAMbeta 1 in the gut was 0.03 transconjugant per recipient cell, and this rate could be simulated in vitro only by forced filter mating. Transfer was not detected in liquid culture matings. Our findings demonstrate that in vitro methods, such as forced filter mating and liquid mating, underestimate the in vivo rates of gene transfer.

* Corresponding author. Mailing address: Department of Agriculture and Environmental Science, King George VI Building, University of Newcastle-upon-Tyne, Newcastle-upon-Tyne NE1 7RU, Great Britain. Phone: 0191 222 5044. Fax: 0191 222 5228. E-mail: trudy.netherwood{at}newcastle.ac.uk.

Applied and Environmental Microbiology, November 1999, p. 5139-5141, Vol. 65, No. 11
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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