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Applied and Environmental Microbiology, November 1999, p. 5177-5181, Vol. 65, No. 11
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Long-Term Survival of Shiga Toxin-Producing Escherichia coli O26, O111, and O157 in Bovine Feces

Hiroshi Fukushima,* Ken Hoshina, and Manabu Gomyoda

Public Health Institute of Shimane Prefecture, Nishihamasada, Matsue, Shimane 690-0122, Japan

Received 24 March 1999/Accepted 17 August 1999

Cattle are an important reservoir of Shiga toxin-producing Escherichia coli (STEC) O26, O111, and O157. The fate of these pathogens in bovine feces at 5, 15, and 25°C was examined. The feces of a cow naturally infected with STEC O26:H11 and two STEC-free cows were studied. STEC O26, O111, and O157 were inoculated into bovine feces at 101, 103, and 105 CFU/g. All three pathogens survived at 5 and 25°C for 1 to 4 weeks and at 15°C for 1 to 8 weeks when inoculated at the low concentration. On samples inoculated with the middle and high concentrations, O26, O111, and O157 survived at 25°C for 3 to 12 weeks, at 15°C for 1 to 18 weeks, and at 5°C for 2 to 14 weeks, respectively. Therefore, these pathogens can survive in feces for a long time, especially at 15°C. The surprising long-term survival of STEC O26, O111, and O157 in bovine feces shows that such feces are a potential vehicle for transmitting not only O157 but also O26 and O111 to cattle, food, and the environment. Appropriate handling of bovine feces is emphasized.

* Corresponding author. Mailing address: Public Health Institute of Shimane Prefecture, 582-1 Nishihamasada, Matsue, Shimane 690-0122, Japan. Phone: 0852-36-8181. Fax: 0852-36-6683. E-mail: hiroshi{at}joho-shimane.or.jp.

Applied and Environmental Microbiology, November 1999, p. 5177-5181, Vol. 65, No. 11
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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