This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shigematsu, T.
Right arrow Articles by Kurane, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shigematsu, T.
Right arrow Articles by Kurane, R.
Agricola
Right arrow Articles by Shigematsu, T.
Right arrow Articles by Kurane, R.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, December 1999, p. 5198-5206, Vol. 65, No. 12
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Soluble Methane Monooxygenase Gene Clusters from Trichloroethylene-Degrading Methylomonas sp. Strains and Detection of Methanotrophs during In Situ Bioremediation

Toru Shigematsu,1,* Satoshi Hanada,1 Masahiro Eguchi,2 Yoichi Kamagata,1 Takahiro Kanagawa,1 and Ryuichiro Kurane1

National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology, Tsukuba, Ibaraki 305-8566,1 and Central Research Laboratories, Organo Corporation, Saitama 335-0015,2 Japan

Received 19 April 1999/Accepted 10 September 1999

The soluble MMO (sMMO) gene clusters from group I methanotrophs were characterized. An 8.1-kb KpnI fragment from Methylomonas sp. strain KSWIII and a 7.5-kb SalI fragment from Methylomonas sp. strain KSPIII which contained the sMMO gene clusters were cloned and sequenced. The sequences of these two fragments were almost identical. The sMMO gene clusters in the fragment consisted of six open reading frames which were 52 to 79% similar to the corresponding genes of previously described sMMO gene clusters of the group II and group X methanotrophs. The phylogenetic analysis of the predicted amino acid sequences of sMMO demonstrated that the sMMOs from these strains were closer to that from M. capsulatus Bath in the group X methanotrophs than to those from Methylosinus trichosporium OB3b and Methylocystis sp. strain M in the group II methanotrophs. Based on the sequence data of sMMO genes of our strains and other methanotrophs, we designed a new PCR primer to amplify sMMO gene fragments of all the known methanotrophs harboring the mmoX gene. The primer set was successfully used for detecting methanotrophs in the groundwater of trichloroethylene-contaminated sites during in situ-biostimulation treatments.

* Corresponding author. Present address: Department of Applied Chemistry and Biochemistry, Faculty of Engineering, Kumamoto University, Kurokami 2-39-1, Kumamoto-City, Kumamoto 860-8555, Japan. Phone: 81-96-342-3668. Fax: 81-96-342-3679. E-mail: shige{at}kumamoto-u.ac.jp.

Applied and Environmental Microbiology, December 1999, p. 5198-5206, Vol. 65, No. 12
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ali, H., Scanlan, J., Dumont, M. G., Murrell, J. C. (2006). Duplication of the mmoX gene in Methylosinus sporium: cloning, sequencing and mutational analysis.. Microbiology 152: 2931-2942 [Abstract] [Full Text]  
  • Erwin, D. P., Erickson, I. K., Delwiche, M. E., Colwell, F. S., Strap, J. L., Crawford, R. L. (2005). Diversity of Oxygenase Genes from Methane- and Ammonia-Oxidizing Bacteria in the Eastern Snake River Plain Aquifer. Appl. Environ. Microbiol. 71: 2016-2025 [Abstract] [Full Text]  
  • Kotani, T., Yamamoto, T., Yurimoto, H., Sakai, Y., Kato, N. (2003). Propane Monooxygenase and NAD+-Dependent Secondary Alcohol Dehydrogenase in Propane Metabolism by Gordonia sp. Strain TY-5. J. Bacteriol. 185: 7120-7128 [Abstract] [Full Text]  
  • Stafford, G. P., Scanlan, J., McDonald, I. R., Murrell, J. C. (2003). rpoN, mmoR and mmoG, genes involved in regulating the expression of soluble methane monooxygenase in Methylosinus trichosporium OB3b. Microbiology 149: 1771-1784 [Abstract] [Full Text]  
  • Csaki, R., Bodrossy, L., Klem, J., Murrell, J. C., Kovacs, K. L. (2003). Genes involved in the copper-dependent regulation of soluble methane monooxygenase of Methylococcus capsulatus (Bath): cloning, sequencing and mutational analysis. Microbiology 149: 1785-1795 [Abstract] [Full Text]  
  • Sluis, M. K., Sayavedra-Soto, L. A., Arp, D. J. (2002). Molecular analysis of the soluble butane monooxygenase from 'Pseudomonas butanovora'. Microbiology 148: 3617-3629 [Abstract] [Full Text]  
  • Heyer, J., Galchenko, V. F., Dunfield, P. F. (2002). Molecular phylogeny of type II methane-oxidizing bacteria isolated from various environments. Microbiology 148: 2831-2846 [Abstract] [Full Text]  
  • Suyama, T., Shigematsu, T., Suzuki, T., Tokiwa, Y., Kanagawa, T., Nagashima, K. V. P., Hanada, S. (2002). Photosynthetic Apparatus in Roseateles depolymerans 61A Is Transcriptionally Induced by Carbon Limitation. Appl. Environ. Microbiol. 68: 1665-1673 [Abstract] [Full Text]  
  • Merkx, M., Lippard, S. J. (2002). Why OrfY?. CHARACTERIZATION OF MMOD, A LONG OVERLOOKED COMPONENT OF THE SOLUBLE METHANE MONOOXYGENASE FROM METHYLOCOCCUS CAPSULATUS (BATH). J. Biol. Chem. 277: 5858-5865 [Abstract] [Full Text]  
  • Auman, A. J., Speake, C. C., Lidstrom, M. E. (2001). nifH Sequences and Nitrogen Fixation in Type I and Type II Methanotrophs. Appl. Environ. Microbiol. 67: 4009-4016 [Abstract] [Full Text]  
  • Horz, H.-P., Yimga, M. T., Liesack, W. (2001). Detection of Methanotroph Diversity on Roots of Submerged Rice Plants by Molecular Retrieval of pmoA, mmoX, mxaF, and 16S rRNA and Ribosomal DNA, Including pmoA-Based Terminal Restriction Fragment Length Polymorphism Profiling. Appl. Environ. Microbiol. 67: 4177-4185 [Abstract] [Full Text]  
  • Auman, A. J., Stolyar, S., Costello, A. M., Lidstrom, M. E. (2000). Molecular Characterization of Methanotrophic Isolates from Freshwater Lake Sediment. Appl. Environ. Microbiol. 66: 5259-5266 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»