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Applied and Environmental Microbiology, December 1999, p. 5322-5327, Vol. 65, No. 12
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Characterization of Unexpected Growth of Escherichia coli O157:H7 by Modeling

Marie Cornu,1,* Marie Laure Delignette-Muller,2 and Jean-Pierre Flandrois1

CNRS UMR 5558, Laboratoire de Bactériologie, Faculté de Médecine Lyon Sud, 69921 Oullins Cedex,1 and Laboratoire d'Ecologie Microbienne et Parasitaire, Ecole Nationale Vétérinaire de Lyon, 69 280 Marcy l'Etoile,2 France

Received 29 March 1999/Accepted 15 September 1999

Modeling of batch kinetics in minimal synthetic medium was used to characterize Escherichia coli O157:H7 growth, which appeared to be different from the exponential growth expected in minimal synthetic medium and observed for E. coli K-12. The turbidimetric kinetics of 14 of the 15 O157:H7 strains tested (93%) were nonexponential, whereas 25 of the 36 other E. coli strains tested (70%) exhibited exponential kinetics. Moreover, the anomaly was almost corrected when the minimal medium was supplemented with methionine. These observations were confirmed with two reference strains by using plate count monitoring. In mixed cultures, E. coli K-12 had a positive effect on E. coli O157:H7 and corrected its growth anomaly. This demonstrated that commensalism occurred, as the growth curve for E. coli K-12 was not affected. The interaction could be explained by an exchange of methionine, as the effect of E. coli K-12 on E. coli O157:H7 appeared to be similar to the effect of methionine.

* Corresponding author. Mailing address: CNRS UMR 5558, Laboratoire de Bactériologie, Faculté de Médecine Lyon Sud, BP 12, 69921 Oullins Cedex, France. Phone: 33 4 78 86 31 67. Fax: 33 4 78 86 31 49. E-mail: cornu{at}biomserv.univ-lyon1.fr.

Applied and Environmental Microbiology, December 1999, p. 5322-5327, Vol. 65, No. 12
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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