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Applied and Environmental Microbiology, December 1999, p. 5403-5408, Vol. 65, No. 12
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Linking Toluene Degradation with Specific Microbial Populations in Soil

Jessica R. Hanson,1 Jennifer L. Macalady,1 David Harris,2 and Kate M. Scow1,*

Department of Land, Air and Water Resources1 and Stable Isotope Facility,2 University of California, Davis, California 95616

Received 12 May 1999/Accepted 27 September 1999

Phospholipid fatty acid (PLFA) analysis of a soil microbial community was coupled with 13C isotope tracer analysis to measure the community's response to addition of 35 µg of [13C]toluene ml of soil solution-1. After 119 h of incubation with toluene, 96% of the incorporated 13C was detected in only 16 of the total 59 PLFAs (27%) extracted from the soil. Of the total 13C-enriched PLFAs, 85% were identical to the PLFAs contained in a toluene-metabolizing bacterium isolated from the same soil. In contrast, the majority of the soil PLFAs (91%) became labeled when the same soil was incubated with [13C]glucose. Our study showed that coupling 13C tracer analysis with PLFA analysis is an effective technique for distinguishing a specific microbial population involved in metabolism of a labeled substrate in complex environments such as soil.

* Corresponding author. Mailing address: Department of Land, Air and Water Resources, 1 Shields Ave., University of California, Davis, CA 95616. Phone: (530) 752-4632. Fax: (530) 752-1552. E-mail: kmscow{at}ucdavis.edu.

Applied and Environmental Microbiology, December 1999, p. 5403-5408, Vol. 65, No. 12
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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