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Applied and Environmental Microbiology, December 1999, p. 5636-5638, Vol. 65, No. 12
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Regiospecific Internal Desaturation of Aliphatic Compounds by a Mutant Rhodococcus Strain

Kenzo Koike,* Katsutoshi Ara, Shigehito Adachi, Hirofumi Takigawa, Hajime Mori, Shigeo Inoue, Yoshiharu Kimura, and Susumu Ito

Tochigi Research Laboratories of Kao Corporation, Ichikai, Haga, Tochigi 321-3497, Japan

Received 12 July 1999/Accepted 4 October 1999

A mutant Rhodococcus strain lacking the ability to utilize 1-chlorohexadecane was found to cis-desaturate aliphatic compounds, such as 1-chlorohexadecane, n-hexadecane, and heptadecanonitrile, yielding corresponding products with a double bond mainly at the ninth carbon from the terminal methyl groups. A new oxidative pathway involving the cis-desaturation step was suggested for alkane utilization by Rhodococcus spp.

* Corresponding author. Present address: Tokyo Laboratories of Kao Corporation, 1-3-2 Bunka, Sumida, Tokyo 131-8501, Japan. Phone: 81-3-5630-9461. Fax: 81-3-5630-9339. E-mail: 381128{at}kastanet.kao.co.jp.

Applied and Environmental Microbiology, December 1999, p. 5636-5638, Vol. 65, No. 12
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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