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Applied and Environmental Microbiology, February 1999, p. 549-552, Vol. 65, No. 2
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Isolation and Characterization of a Mycobacterium Species Capable of Degrading Three- and Four-Ring Aromatic and Aliphatic Hydrocarbons

Sharon A. Churchill,1,* Jennifer P. Harper,2 and Perry F. Churchill2

Department of Civil and Environmental Engineering, Washington State University, Pullman, Washington 99164-2910,1 and Department of Biological Sciences, University of Alabama, Tuscaloosa, Alabama 35487-03442

Received 8 May 1998/Accepted 20 November 1998

Mycobacterium sp. strain CH1 was isolated from polycyclic aromatic hydrocarbon (PAH)-contaminated freshwater sediments and identified by analysis of 16S rDNA sequences. Strain CH1 was capable of mineralizing three- and four-ring PAHs including phenanthrene, pyrene, and fluoranthene. In addition, strain CH1 could utilize phenanthrene or pyrene as a sole carbon and energy source. A lag phase of at least 3 days was observed during pyrene mineralization. This lag phase decreased to less than 1 day when strain CH1 was grown in the presence of phenanthrene or fluoranthene. Strain CH1 also was capable of using a wide range of alkanes as sole carbon and energy sources. No DNA hybridization was detected with the nahAc gene probe, indicating that enzymes involved in PAH metabolism are not related to the well-characterized naphthalene dioxygenase gene. DNA hybridization was not detected when the alkB gene from Pseudomonas oleovorans was used under high-stringency conditions. However, there was slight but detectable hybridization under low-stringency conditions. This suggests a distant relationship between genes involved in alkane oxidation.

* Corresponding author. Mailing address: Department of Civil and Environmental Engineering, Washington State University, Pullman, WA 99164-2910. Phone: (509) 335-3227. Fax: (509) 335-7632. E-mail: trouble{at}wsu.edu.

Applied and Environmental Microbiology, February 1999, p. 549-552, Vol. 65, No. 2
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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