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Applied and Environmental Microbiology, February 1999, p. 759-765, Vol. 65, No. 2
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Alterations in Adhesion, Transport, and Membrane Characteristics in an Adhesion-Deficient Pseudomonad

M. F. DeFlaun,1 S. R. Oppenheimer,2,dagger S. Streger,1 C. W. Condee,1 and M. Fletcher2,*

Envirogen, Inc., Princeton Research Center, Lawrenceville, New Jersey 08648-4702,1 and Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 212022

Received 23 December 1997/Accepted 8 November 1998

A stable adhesion-deficient mutant of Burkholderia cepacia G4, a soil pseudomonad, was selected in a sand column assay. This mutant (ENV435) was compared to the wild-type strain by examining the adhesion of the organisms to silica sand and their transport through two aquifer sediments that differed in their sand, silt, and clay contents. We compared the longitudinal transport of the wild type and the adhesion mutant to the transport of a conservative chloride tracer in 25-cm-long glass columns. The transport of the wild-type strain was severely retarded compared to the transport of the conservative tracer in a variety of aquifer sediments, while the adhesion mutant and the conservative tracer traveled at similar rates. An intact sediment core study produced similar results; ENV435 was transported at a faster rate and in much greater numbers than G4. The results of hydrophobic interaction chromatography revealed that G4 was significantly more hydrophobic than ENV435, and polyacrylamide gel electrophoresis revealed significant differences in the lipopolysaccharide O-antigens of the adhesion mutant and the wild type. Differences in this cell surface polymer may explain the decreased adhesion of strain ENV435.

* Corresponding author. Present address: Belle W. Baruch Institute for Marine Biology and Coastal Research, University of South Carolina, Columbia, SC 29208. Phone: (803) 777-5288. Fax: (803) 777-3935. E-mail: fletcher{at}biol.sc.edu.

dagger Present address: Osiris Therapeutics, Inc., Baltimore, MD 21231.

Applied and Environmental Microbiology, February 1999, p. 759-765, Vol. 65, No. 2
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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