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Applied and Environmental Microbiology, February 1999, p. 841-845, Vol. 65, No. 2
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Evaluation of Immunomagnetic Separation for Recovery of Infectious Cryptosporidium parvum Oocysts from Environmental Samples

Paul A. Rochelle,* Ricardo De Leon, Anne Johnson, Mic H. Stewart, and Roy L. Wolfe

Water Quality Laboratory, Metropolitan Water District of Southern California, La Verne, California 91750-3399

Received 3 August 1998/Accepted 30 October 1998

Two commercial immunomagnetic separation (IMS) kits for Cryptosporidium were compared for recovery of oocysts from environmental samples. Oocyst recovery efficiencies with the Dynal and Crypto-Scan kits ranged from 62 to 100% and 34 to 74%, respectively, for seeded environmental water concentrates (turbidity of 210 to 11,480 nephelometric turbidity units). Recovery efficiencies were dependent on the mechanism of agitation during the magnetic capture procedure. An assay combining in vitro cell culture and reverse transcriptase PCR demonstrated that oocysts recovered by IMS retained their infectivity.

* Corresponding author. Mailing address: Water Quality Laboratory, Metropolitan Water District of Southern California, 700 Moreno Ave., La Verne, CA 91750-3399. Phone: (909) 392-5155. Fax: (909) 392-5246. E-mail: prochelle{at}mwd.dst.ca.us.

Applied and Environmental Microbiology, February 1999, p. 841-845, Vol. 65, No. 2
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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