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Applied and Environmental Microbiology, March 1999, p. 1316-1319, Vol. 65, No. 3
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Heat Resistance of Native and Demineralized Spores of Bacillus subtilis Sporulated at Different Temperatures

Alfredo Palop, Francisco J. Sala, and Santiago Condón*

Tecnología de los Alimentos, Universidad de Zaragoza, Zaragoza, Spain

Received 5 October 1998/Accepted 17 December 1998

Demineralization reduced heat resistance of B. subtilis spores, but the pattern and magnitude of the reduction depended on sporulation temperature and on heating menstruum pH. The differences in heat resistance of native spores caused by sporulation temperature almost disappeared after demineralization. Demineralized spores were still susceptible to the heat-sensitizing effect of acidic pH.

* Corresponding author. Mailing address: Tecnología de los Alimentos, Facultad de Veterinaria, C/ Miguel Servet, 177, 50013 Zaragoza, Spain. Phone: 34 976 76 1581. Fax: 34 976 76 15 90. E-mail: scondon{at}posta.unizar.es.

Applied and Environmental Microbiology, March 1999, p. 1316-1319, Vol. 65, No. 3
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.




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