This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chen, P.
Right arrow Articles by Caufield, P. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, P.
Right arrow Articles by Caufield, P. W.
Agricola
Right arrow Articles by Chen, P.
Right arrow Articles by Caufield, P. W.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, March 1999, p. 1356-1360, Vol. 65, No. 3
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The Specific Genes for Lantibiotic Mutacin II Biosynthesis in Streptococcus mutans T8 Are Clustered and Can Be Transferred En Bloc

Ping Chen, FengXia Qi, Jan Novak, and Page W. Caufield*

Department of Oral Biology, School of Dentistry, University of Alabama at Birmingham, Birmingham, Alabama 35294

Received 3 September 1998/Accepted 14 December 1998

Mutacin II is a ribosomally synthesized peptide lantibiotic produced by group II Streptococcus mutans. DNA sequencing has revealed that the mutacin II biosynthetic gene cluster consists of seven specific open reading frames: a regulator (mutR), the prepromutacin structural gene (mutA), a modifying protein (mutM), an ABC transporter (mutT), and an immunity cluster (mutFEG). Transformations of a non-mutacin-producing strain, S. mutans UA159, and a mutacin I-producing strain, S. mutans UA140, with chromosomal DNA from S. mutans T8 with an aphIII marker inserted upstream of the mutacin II structural gene yielded transformants producing mutacin II and mutacins I and II, respectively.

* Corresponding author. Mailing address: LHRB 246, Department of Oral Biology, School of Dentistry, University of Alabama at Birmingham, 1919 7th Ave. South, Birmingham, AL 35294. Phone: (205) 934-4327. Fax: (205) 975-6773. E-mail: page{at}uab.edu.

Applied and Environmental Microbiology, March 1999, p. 1356-1360, Vol. 65, No. 3
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Okuda, K.-i., Yanagihara, S., Shioya, K., Harada, Y., Nagao, J.-i., Aso, Y., Zendo, T., Nakayama, J., Sonomoto, K. (2008). Binding Specificity of the Lantibiotic-Binding Immunity Protein NukH. Appl. Environ. Microbiol. 74: 7613-7619 [Abstract] [Full Text]  
  • Chong, P., Drake, L., Biswas, I. (2008). LiaS Regulates Virulence Factor Expression in Streptococcus mutans. Infect. Immun. 76: 3093-3099 [Abstract] [Full Text]  
  • Okuda, K.-i., Aso, Y., Nakayama, J., Sonomoto, K. (2008). Cooperative Transport between NukFEG and NukH in Immunity against the Lantibiotic Nukacin ISK-1 Produced by Staphylococcus warneri ISK-1. J. Bacteriol. 190: 356-362 [Abstract] [Full Text]  
  • Kreth, J., Hung, D. C. I., Merritt, J., Perry, J., Zhu, L., Goodman, S. D., Cvitkovitch, D. G., Shi, W., Qi, F. (2007). The response regulator ComE in Streptococcus mutans functions both as a transcription activator of mutacin production and repressor of CSP biosynthesis. Microbiology 153: 1799-1807 [Abstract] [Full Text]  
  • Cotter, P. D., Draper, L. A., Lawton, E. M., McAuliffe, O., Hill, C., Ross, R. P. (2006). Overproduction of wild-type and bioengineered derivatives of the lantibiotic lacticin 3147.. Appl. Environ. Microbiol. 72: 4492-4496 [Abstract] [Full Text]  
  • Waterhouse, J. C., Russell, R. R. B. (2006). Dispensable genes and foreign DNA in Streptococcus mutans. Microbiology 152: 1777-1788 [Abstract] [Full Text]  
  • Eckert, R., Qi, F., Yarbrough, D. K., He, J., Anderson, M. H., Shi, W. (2006). Adding Selectivity to Antimicrobial Peptides: Rational Design of a Multidomain Peptide against Pseudomonas spp.. Antimicrob. Agents Chemother. 50: 1480-1488 [Abstract] [Full Text]  
  • Hale, J. D. F., Ting, Y.-T., Jack, R. W., Tagg, J. R., Heng, N. C. K. (2005). Bacteriocin (Mutacin) Production by Streptococcus mutans Genome Sequence Reference Strain UA159: Elucidation of the Antimicrobial Repertoire by Genetic Dissection. Appl. Environ. Microbiol. 71: 7613-7617 [Abstract] [Full Text]  
  • Hale, J. D. F., Heng, N. C. K., Jack, R. W., Tagg, J. R. (2005). Identification of nlmTE, the Locus Encoding the ABC Transport System Required for Export of Nonlantibiotic Mutacins in Streptococcus mutans. J. Bacteriol. 187: 5036-5039 [Abstract] [Full Text]  
  • Stein, T., Heinzmann, S., Dusterhus, S., Borchert, S., Entian, K.-D. (2005). Expression and Functional Analysis of the Subtilin Immunity Genes spaIFEG in the Subtilin-Sensitive Host Bacillus subtilis MO1099. J. Bacteriol. 187: 822-828 [Abstract] [Full Text]  
  • Wang, B.-Y., Kuramitsu, H. K. (2005). Interactions between Oral Bacteria: Inhibition of Streptococcus mutans Bacteriocin Production by Streptococcus gordonii. Appl. Environ. Microbiol. 71: 354-362 [Abstract] [Full Text]  
  • Uguen, P., Hindre, T., Didelot, S., Marty, C., Haras, D., Le Pennec, J.-P., Vallee-Rehel, K., Dufour, A. (2005). Maturation by LctT Is Required for Biosynthesis of Full-Length Lantibiotic Lacticin 481. Appl. Environ. Microbiol. 71: 562-565 [Abstract] [Full Text]  
  • Merritt, J., Qi, F., Shi, W. (2005). A unique nine-gene comY operon in Streptococcus mutans. Microbiology 151: 157-166 [Abstract] [Full Text]  
  • Merritt, J., Qi, F., Goodman, S. D., Anderson, M. H., Shi, W. (2003). Mutation of luxS Affects Biofilm Formation in Streptococcus mutans. Infect. Immun. 71: 1972-1979 [Abstract] [Full Text]  
  • Sanchez-Hidalgo, M., Maqueda, M., Galvez, A., Abriouel, H., Valdivia, E., Martinez-Bueno, M. (2003). The Genes Coding for Enterocin EJ97 Production by Enterococcus faecalis EJ97 Are Located on a Conjugative Plasmid. Appl. Environ. Microbiol. 69: 1633-1641 [Abstract] [Full Text]  
  • Diaz, M., Valdivia, E., Martinez-Bueno, M., Fernandez, M., Soler-Gonzalez, A. S., Ramirez-Rodrigo, H., Maqueda, M. (2003). Characterization of a New Operon, as-48EFGH, from the as-48 Gene Cluster Involved in Immunity to Enterocin AS-48. Appl. Environ. Microbiol. 69: 1229-1236 [Abstract] [Full Text]  
  • Ajdic', D., McShan, W. M., McLaughlin, R. E., Savic', G., Chang, J., Carson, M. B., Primeaux, C., Tian, R., Kenton, S., Jia, H., Lin, S., Qian, Y., Li, S., Zhu, H., Najar, F., Lai, H., White, J., Roe, B. A., Ferretti, J. J. (2002). Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen. Proc. Natl. Acad. Sci. USA 99: 14434-14439 [Abstract] [Full Text]  
  • Scupham, A. J., Dong, Y., Triplett, E. W. (2002). Role of tfxE, but Not tfxG, in Trifolitoxin Resistance. Appl. Environ. Microbiol. 68: 4334-4340 [Abstract] [Full Text]  
  • Guder, A., Schmitter, T., Wiedemann, I., Sahl, H.-G., Bierbaum, G. (2002). Role of the Single Regulator MrsR1 and the Two-Component System MrsR2/K2 in the Regulation of Mersacidin Production and Immunity. Appl. Environ. Microbiol. 68: 106-113 [Abstract] [Full Text]  
  • Dufour, A., Rincé, A., Uguen, P., Le Pennec, J.-P. (2000). IS1675, a Novel Lactococcal Insertion Element, Forms a Transposon-Like Structure Including the Lacticin 481 Lantibiotic Operon. J. Bacteriol. 182: 5600-5605 [Abstract] [Full Text]  
  • Uguen, P., Le Pennec, J.-P., Dufour, A. (2000). Lantibiotic Biosynthesis: Interactions between Prelacticin 481 and Its Putative Modification Enzyme, LctM. J. Bacteriol. 182: 5262-5266 [Abstract] [Full Text]  
  • Qi, F., Chen, P., Caufield, P. W. (2000). Purification and Biochemical Characterization of Mutacin I from the Group I Strain of Streptococcus mutans, CH43, and Genetic Analysis of Mutacin I Biosynthesis Genes. Appl. Environ. Microbiol. 66: 3221-3229 [Abstract] [Full Text]  
  • Krull, R. E., Chen, P., Novak, J., Kirk, M., Barnes, S., Baker, J., Krishna, N. R., Caufield, P. W. (2000). Biochemical Structural Analysis of the Lantibiotic Mutacin II. J. Biol. Chem. 275: 15845-15850 [Abstract] [Full Text]  
  • Qi, F., Chen, P., Caufield, P. W. (1999). Purification of Mutacin III from Group III Streptococcus mutans UA787 and Genetic Analyses of Mutacin III Biosynthesis Genes. Appl. Environ. Microbiol. 65: 3880-3887 [Abstract] [Full Text]  
  • Marco-Noales, E., Biosca, E. G., Amaro, C. (1999). Effects of Salinity and Temperature on Long-Term Survival of the Eel Pathogen Vibrio vulnificus Biotype 2 (Serovar E). Appl. Environ. Microbiol. 65: 1117-1126 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»