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Applied and Environmental Microbiology, April 1999, p. 1806-1810, Vol. 65, No. 4
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Genetic Analysis of Biodegradation of Tetralin by a Sphingomonas Strain

María José Hernáez,1 Walter Reineke,2 and Eduardo Santero1,*

Departamento de Genética, Facultad de Biología, Universidad de Sevilla, E-41080 Seville, Spain,1 and Chemische Mikrobiologie, Bergische Universität-Gesamthochschule Wuppertal, D-42097 Wuppertal, Germany2

Received 1 February 1999/Accepted 3 February 1999

A strain designated TFA which very efficiently utilizes tetralin has been isolated from the Rhine river. The strain has been identified as Sphingomonas macrogoltabidus, based on 16S rDNA sequence similarity. Genetic analysis of tetralin biodegradation has been performed by insertion mutagenesis and by physical analysis and analysis of complementation between the mutants. The genes involved in tetralin utilization are clustered in a region of 9 kb, comprising at least five genes grouped in two divergently transcribed operons.

* Corresponding author. Mailing address: Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Ap. 1095, E-41080 Sevilla, Spain. Phone: 34-95-4557106. Fax: 34-95-4557104. E-mail: esantero{at}cica.es.

Applied and Environmental Microbiology, April 1999, p. 1806-1810, Vol. 65, No. 4
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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