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Applied and Environmental Microbiology, May 1999, p. 2238-2242, Vol. 65, No. 5
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Detection of Bacteriocins by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Natisha L. Rose, Peter Sporns, and Lynn M. McMullen*

Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada

Received 18 September 1998/Accepted 8 February 1999

The use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the detection of bacteriocins was investigated. A 30-s water wash of the sample on the MALDI-TOF MS probe was effective in removing contaminants of the analyte. This method was used for rapid detection of nisin, pediocin, brochocin A and B, and enterocin A and B from culture supernatants and for detection of enterocin B throughout its purification.

* Corresponding author. Mailing address: 4-10 Agriculture/Forestry Centre, University of Alberta, Edmonton, Alberta T6G 2P5, Canada. Phone: 780-492-6015. Fax: 780-492-8914. E-mail: lynn.mcmullen{at}ualberta.ca.

Applied and Environmental Microbiology, May 1999, p. 2238-2242, Vol. 65, No. 5
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Garneau, S., Ference, C. A., van Belkum, M. J., Stiles, M. E., Vederas, J. C. (2003). Purification and Characterization of Brochocin A and Brochocin B(10-43), a Functional Fragment Generated by Heterologous Expression in Carnobacterium piscicola. Appl. Environ. Microbiol. 69: 1352-1358 [Abstract] [Full Text]  
  • Franz, C. M. A. P., Worobo, R. W., Quadri, L. E. N., Schillinger, U., Holzapfel, W. H., Vederas, J. C., Stiles, M. E. (1999). Atypical Genetic Locus Associated with Constitutive Production of Enterocin B by Enterococcus faecium BFE 900. Appl. Environ. Microbiol. 65: 2170-2178 [Abstract] [Full Text]  


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