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Applied and Environmental Microbiology, May 1999, p. 2250-2252, Vol. 65, No. 5
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

A Sandwiched-Culture Technique for Evaluation of Heterologous Protein Production in a Filamentous Fungus

Sigrídur A. Ásgeirsdóttir,* Karin Scholtmeijer, and Joseph G. H. Wessels

Molecular Plant Biology Laboratory, Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9751 NN Haren, The Netherlands

Received 12 November 1998/Accepted 1 March 1999

Aspergillus niger is known for its efficient excretion machinery. However, problems have often arisen in obtaining high amounts of heterologous proteins in the culture medium. Here we present a quick method using sandwiched colonies to evaluate transgenic strains for secretion of heterologous proteins. Expressing the ABH1 hydrophobin of Agaricus bisporus in A. niger, we showed that low production levels of the heterologous protein are probably due to extracellular proteolytic degradation of the protein.

* Corresponding author. Present address: Department of Pharmacokinetics and Drug Delivery, University Center for Pharmacy, University of Groningen, A. Deusinglaan 1, 9713 AV Groningen, The Netherlands. Phone: 31-50-3633292. Fax: 31-50-3633247. E-mail: s.asgeirsdottir{at}farm.rug.nl.

Applied and Environmental Microbiology, May 1999, p. 2250-2252, Vol. 65, No. 5
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.




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