This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chopra, R.
Right arrow Articles by Ganesan, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chopra, R.
Right arrow Articles by Ganesan, K.
Agricola
Right arrow Articles by Chopra, R.
Right arrow Articles by Ganesan, K.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, May 1999, p. 2267-2268, Vol. 65, No. 5
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Elevated Growth of Saccharomyces cerevisiae ATH1 Null Mutants on Glucose Is an Artifact of Nonmatching Auxotrophies of Mutant and Reference Strainsdagger

Rohini Chopra, Vishva Mitra Sharma, and K. Ganesan*

Institute of Microbial Technology, Sector 39A, Chandigarh 160 036, India

Received 26 May 1998/Accepted 21 January 1999

Yeast strains disrupted for ATH1, which encodes vacuolar acid trehalase, have been reported to grow to higher cell densities than reference strains. We showed that the increase in cell density is due to the URA3 gene introduced as a part of the disruption and concluded that the misinterpretation is a result of not using a control strain with matching auxotrophic markers.

* Corresponding author. Mailing address: Institute of Microbial Technology, Sector 39A, Chandigarh 160 036, India. Phone: 0091-172-695215, -695216, or -695219, ext. 442. Fax: 0091-172-690585 or -690632. E-mail: ganesank{at}usa.net.

dagger Communication no. 007/98 of the Institute of Microbial Technology, Chandigarh, India.

Applied and Environmental Microbiology, May 1999, p. 2267-2268, Vol. 65, No. 5
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kaino, T., Tateiwa, T., Mizukami-Murata, S., Shima, J., Takagi, H. (2008). Self-Cloning Baker's Yeasts That Accumulate Proline Enhance Freeze Tolerance in Doughs. Appl. Environ. Microbiol. 74: 5845-5849 [Abstract] [Full Text]  
  • Hohmann, S. (2002). Osmotic Stress Signaling and Osmoadaptation in Yeasts. Microbiol. Mol. Biol. Rev. 66: 300-372 [Abstract] [Full Text]  
  • Pronk, J. T. (2002). Auxotrophic Yeast Strains in Fundamental and Applied Research. Appl. Environ. Microbiol. 68: 2095-2100 [Full Text]  
  • de Vries, R. P., Visser, J. (1999). Regulation of the Feruloyl Esterase (faeA) Gene from Aspergillus niger. Appl. Environ. Microbiol. 65: 5500-5503 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»