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Applied and Environmental Microbiology, June 1999, p. 2650-2653, Vol. 65, No. 6
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

PCR Comparison of Mycobacterium avium Isolates Obtained from Patients and Foods

S. Yoder,1,2,dagger ,* C. Argueta,1,2 A. Holtzman,1 T. Aronson,1 O. G. W. Berlin,1 P. Tomasek,2 N. Glover,3 S. Froman,1 and G. Stelma Jr.4

Education and Research Institute1 and Department of Pathology,3 Olive View---UCLA Medical Center, Sylmar, California 91342; Department of Biology, California State University, Northridge, Northridge, California 913302; and U.S. Environmental Protection Agency, Cincinnati, Ohio 452684

Received 8 September 1998/Accepted 18 March 1999

Mycobacterium avium is a cause of disseminated disease in AIDS patients. A need for a better understanding of possible sources and routes of transmission of this organism has arisen. This study utilized a PCR typing method designed to amplify DNA segments located between the insertion sequences IS1245 and IS1311 to compare levels of relatedness of M. avium isolates found in patients and foods. Twenty-five of 121 food samples yielded 29 mycobacterial isolates, of which 12 were M. avium. Twelve food and 103 clinical M. avium isolates were tested. A clinical isolate was found to be identical to a food isolate, and close relationships were found between two patient isolates and two food isolates. Relatedness between food isolates and patient isolates suggests the possibility that food is a potential source of M. avium infection. This study demonstrates a rapid, inexpensive method for typing M. avium, possibly replacing pulsed-field gel electrophoresis.

* Corresponding author. Mailing address: Olive View---UCLA Education and Research Institute, Old Lab Room 218, 14445 Olive View Dr., Sylmar, CA 91342. Phone: (818) 364-3449. Fax: (818) 364-3465. E-mail: sean.yoder{at}csun.edu.

dagger Present address: Section of Periodontics, UCLA School of Dentistry, Los Angeles, CA 90025-1668.

Applied and Environmental Microbiology, June 1999, p. 2650-2653, Vol. 65, No. 6
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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