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Applied and Environmental Microbiology, June 1999, p. 2730-2737, Vol. 65, No. 6
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Modulation of Lipid Metabolism and Spiramycin Biosynthesis in Streptomyces ambofaciens Unstable Mutants

Catherine Schauner,1 Annie Dary,2,* Ahmed Lebrihi,1,dagger Pierre Leblond,2 Bernard Decaris,2 and Pierre Germain1

Laboratoire de Fermentations et de Bioconversions Industrielles, ENSAIA, Institut National Polytechnique de Lorraine,1 and Laboratoire de Génétique et de Microbiologie, Associé à l'Institut National de la Recherche Agronomique, Faculté des Sciences de l'Université Henri Poincaré, Nancy 1,2 Vandoeuvre-lès-Nancy, France

Received 19 October 1998/Accepted 3 March 1999

Streptomyces ambofaciens is prone to genetic instability involving genomic rearrangements at the extremities of the chromosomal DNA. An amplified DNA sequence (ADS205), including an open reading frame (orfPS), is responsible for the reversible loss of spiramycin production in the mutant strain NSA205 (ADS205+ Spi-). The product of orfPS is homologous to polyketide synthase systems (PKSs) involved in the biosynthesis of erythromycin and rapamycin and is overexpressed in strain NSA205 compared with the parental strain RP181110. As PKSs and fatty acid synthase systems have the same precursors, we tested the possibility that overexpression of orfPS also affects lipid metabolism in strain NSA205. This report focuses on comparative analysis of lipids in strain RP181110, the mutant strain NSA205, and a derivative, NSA228 (ADS205- Spi+). NSA205 showed a dramatically depressed lipid content consisting predominantly of phospholipids and triacylglycerols. This lipid content was globally restored in strain NSA228, which had lost ADS205. Furthermore, strains RP181110 and NSA205 presented similar phospholipid and triacylglycerol compositions. No abnormal fatty acids were detected in NSA205.

* Corresponding author. Mailing address: Laboratoire de Génétique et de Microbiologie, Associé à l'Institut National de la Recherche Agronomique, Faculté des Sciences de l'Université Henri Poincaré, Nancy 1, BP 239, F-54506 Vandoeuvre-lès-Nancy, France. Phone: (33) 3 83 91 21 79. Fax: (33) 3 83 91 25 00. E-mail: dary{at}scbiol.u-nancy.fr.

dagger Present address: Equipe Microbiologie et Hygiène Alimentaire, ENSAT, Institut National Polytechnique de Toulouse, F-31326 Castanet Tolosan, France.

Applied and Environmental Microbiology, June 1999, p. 2730-2737, Vol. 65, No. 6
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Pang, X., Aigle, B., Girardet, J.-M., Mangenot, S., Pernodet, J.-L., Decaris, B., Leblond, P. (2004). Functional Angucycline-Like Antibiotic Gene Cluster in the Terminal Inverted Repeats of the Streptomyces ambofaciens Linear Chromosome. Antimicrob. Agents Chemother. 48: 575-588 [Abstract] [Full Text]  


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