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Applied and Environmental Microbiology, June 1999, p. 2745-2747, Vol. 65, No. 6
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

High-Level Production of Heterologous Protein by Engineered Yeasts Grown in Cottage Cheese Whey

Carlo Maullu,1 Giorgio Lampis,1,* Alessandra Desogus,1 Angela Ingianni,1 Gian Maria Rossolini,2 and Raffaello Pompei1

Dipartimento di Scienze Mediche, Cattedra di Microbiologia Applicata, Università di Cagliari, 09124 Cagliari,1 and Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, 53100 Siena,2 Italy

Received 9 December 1998/Accepted 8 March 1999

Cottage cheese whey is a cheese industry by-product still rich in proteins and lactose. Its recycling is seldom cost-effective. In this work we show that the lactose-utilizing yeast Kluyveromyces lactis, engineered for production of recombinant human lysozyme, can be grown in cottage cheese whey, resulting in high-level production of the heterologous protein (125 µg/ml).

* Corresponding author. Mailing address: Cattedra di Microbiologia Applicata, Università di Cagliari, via Porcell 4, 09124 Cagliari, Italy. Phone: 39-070-6758481. Fax: 39-070-6758482. E-mail: glampis{at}unica.it.

Applied and Environmental Microbiology, June 1999, p. 2745-2747, Vol. 65, No. 6
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Raimondi, S., Zanni, E., Talora, C., Rossi, M., Palleschi, C., Uccelletti, D. (2008). SOD1, a New Kluyveromyces lactis Helper Gene for Heterologous Protein Secretion. Appl. Environ. Microbiol. 74: 7130-7137 [Abstract] [Full Text]  


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