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Applied and Environmental Microbiology, July 1999, p. 3158-3163, Vol. 65, No. 7
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Formation of Hyodeoxycholic Acid from Muricholic Acid and Hyocholic Acid by an Unidentified Gram-Positive Rod Termed HDCA-1 Isolated from Rat Intestinal Microflora

H. J. Eyssen, G. De Pauw, and J. Van Eldere*

Rega Institute for Medical Research, Katholieke Universiteit Leuven, Leuven, Belgium

Received 18 February 1999/Accepted 6 May 1999

From the rat intestinal microflora we isolated a gram-positive rod, termed HDCA-1, that is a member of a not previously described genomic species and that is able to transform the 3alpha ,6beta ,7beta -trihydroxy bile acid beta -muricholic acid into hyodeoxycholic acid (3alpha ,6alpha -dihydroxy acid) by dehydroxylation of the 7beta -hydroxy group and epimerization of the 6beta -hydroxy group into a 6alpha -hydroxy group. Other bile acids that were also transformed into hyodeoxycholic acid were hyocholic acid (3alpha ,6alpha ,7alpha -trihydroxy acid), alpha -muricholic acid (3alpha ,6beta ,7alpha -trihydroxy acid), and omega -muricholic acid (3alpha ,6alpha ,7beta -trihydroxy acid). The strain HDCA-1 could not be grown unless a nonconjugated 7-hydroxylated bile acid and an unidentified growth factor produced by a Ruminococcus productus strain that was also isolated from the intestinal microflora were added to the culture medium. Germfree rats selectively associated with the strain HDCA-1 plus a bile acid-deconjugating strain and the growth factor-producing R. productus strain converted beta -muricholic acid almost completely into hyodeoxycholic acid.

* Corresponding author. Mailing address: Rega Institute, Minderbroederstraat 10, B-3000 Leuven, Belgium. Phone: 16 33 73 72. Fax: 16 33 73 40. E-mail: johan.vaneldere{at}rega.kuleuven.ac.be.

Applied and Environmental Microbiology, July 1999, p. 3158-3163, Vol. 65, No. 7
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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