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Applied and Environmental Microbiology, July 1999, p. 3236-3239, Vol. 65, No. 7
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Comparison of Sensitivity of Immunofluorescent Microscopy to That of a Combination of Immunofluorescent Microscopy and Immunomagnetic Separation for Detection of Cryptosporidium parvum Oocysts in Adult Bovine Feces

Maria das Graças C. Pereira, Edward R. Atwill,* and Ted Jones

Veterinary Medicine Teaching and Research Center, School of Veterinary Medicine, University of California, Davis, Tulare, California 93274

Received 21 October 1998/Accepted 6 April 1999

A direct immunofluorescence assay (DFA) (Merifluor; Meridian Diagnostics, Inc., Cincinnati, Ohio) was compared to an immunomagnetic separation (IMS) assay (Dynabeads; Dynal, Inc., Lake Success, N.Y.) coupled with immunofluorescent microscopy (Waterborne, Inc., New Orleans, La.) for their ability to detect low concentrations of Cryptosporidium parvum oocysts in adult bovine fecal material. IMS-DFA resulted in a 2-log-unit increase in sensitivity (10 oocysts/g) compared to DFA alone (1,000 oocysts/g). The higher sensitivity obtained with IMS-DFA resulted from testing 2 g of fecal material instead of the 13 to 19 mg of fecal material tested in the DFA; the increased sensitivity was not attributable to a higher percent recovery.

* Corresponding author. Mailing address: Veterinary Medicine Teaching and Research Center, School of Veterinary Medicine, University of California, Davis, 18830 Rd. 112, Tulare, CA 93274. Phone: (559) 688-1731. Fax: (559) 686-4231. E-mail: ratwill{at}vmtrc.ucdavis.edu.

Applied and Environmental Microbiology, July 1999, p. 3236-3239, Vol. 65, No. 7
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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