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Applied and Environmental Microbiology, July 1999, p. 3258-3260, Vol. 65, No. 7
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Influence of 1-[(E)-2-(2-Methyl-4-Nitrophenyl)Diaz-1-enyl]Pyrrolidine- 2-Carboxylic Acid and Diphenyliodonium Chloride on Ruminal Protein Metabolism and Ruminal Microorganisms

Fabienne Floret,1 Lal C. Chaudhary,2 William C. Ellis,3 Suliman El Hassan,4 Nest McKain,1 C. James Newbold,1 and R. John Wallace1,*

Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, United Kingdom1; Indian Veterinary Research Institute, Izatnagar-243 122, India2; Department of Animal Science, Texas A & M University, College Station, Texas 778433; and Physiology Department, Faculty of Veterinary Medicine, University of Khartoum, Khartoum North, Sudan4

Received 1 February 1999/Accepted 3 May 1999

The effects of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine-2-carboxylic acid (LY29) and diphenyliodonium chloride (DIC) on the degradation of protein to ammonia were determined in a mixed rumen microbial population taken from sheep on a grass hay-concentrate diet. Both compounds decreased NH3 production by inhibiting deamination of amino acids. LY29, but not DIC, inhibited growth of the high-activity ammonia-producing species, Clostridium aminophilum and Clostridium sticklandii.


* Corresponding author. Mailing address: Rowett Research Institute, Bucksburn, Aberdeen, AB21 9SB, United Kingdom. Phone: 44 1224 716656. Fax: 44 1224 716687. E-mail: RJW{at}RRI.SARI.AC.UK.


Applied and Environmental Microbiology, July 1999, p. 3258-3260, Vol. 65, No. 7
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.