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Applied and Environmental Microbiology, August 1999, p. 3401-3406, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Differentiation of Fusarium subglutinans f. sp. pini by Histone Gene Sequence Data

E. T. Steenkamp,1,* B. D. Wingfield,1 T. A. Coutinho,1 M. J. Wingfield,1 and W. F. O. Marasas2

Tree Pathology Co-operative Programme, Forestry and Agricultural Biotechnology Institute, Departments of Genetics, Microbiology and Plant Pathology, University of Pretoria, Pretoria 0002,1 and Programme on Mycotoxins and Experimental Carcinogenesis, Medical Research Council, Tygerberg 7050,2 South Africa

Received 9 February 1999/Accepted 28 May 1999

Fusarium subglutinans f. sp. pini (= F. circinatum) is a pathogen of pine and is one of eight mating populations (i.e., biological species) in the Gibberella fujikuroi species complex. This species complex includes F. thapsinum, F. moniliforme (= F. verticillioides), F. nygamai, and F. proliferatum, as well as F. subglutinans associated with sugarcane, maize, mango, and pineapple. Differentiating these forms of F. subglutinans usually requires pathogenicity tests, which are often time-consuming and inconclusive. Our objective was to develop a technique to differentiate isolates of F. subglutinans f. sp. pini from other isolates identified as F. subglutinans. We sequenced the histone H3 gene from a representative set of Fusarium isolates. The H3 gene sequence was conserved and contained two introns in all the isolates studied. From both the intron and the exon sequence data, we developed a PCR-restriction fragment length polymorphism technique that reliably distinguishes F. subglutinans f. sp. pini from the other biological species in the G. fujikuroi species complex.

* Corresponding author. Mailing address: Tree Pathology Co-operative Programme, Forestry and Agricultural Biotechnology Institute, University of Pretoria, 74 Lunnon Rd., Hillcrest, Pretoria 0002, South Africa. Phone: (27 12) 420-3948. Fax: (27 12) 420-3947. E-mail: emma.steenkamp{at}fabi.up.ac.za.

Applied and Environmental Microbiology, August 1999, p. 3401-3406, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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