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Applied and Environmental Microbiology, August 1999, p. 3627-3632, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Seasonal Variations in Microbial Populations and Environmental Conditions in an Extreme Acid Mine Drainage Environment

Katrina J. Edwards,* Thomas M. Gihring, and Jillian F. Banfield

Department of Geology and Geophysics, University of Wisconsin---Madison, Madison, Wisconsin 53706

Received 20 October 1998/Accepted 3 March 1999

Microbial populations, their distributions, and their aquatic environments were studied over a year (1997) at an acid mine drainage (AMD) site at Iron Mountain, Calif. Populations were quantified by fluorescence in situ hybridizations with group-specific probes. Probes were used for the domains Eucarya, Bacteria, and Archaea and the two species most widely studied and implicated for their role in AMD production, Thiobacillus ferrooxidans and Leptospirillum ferrooxidans. Results show that microbial populations, in relative proportions and absolute numbers, vary spatially and seasonally and correlate with geochemical and physical conditions (pH, temperature, conductivity, and rainfall). Bacterial populations were in the highest proportion (>95%) in January. Conversely, archaeal populations were in the highest proportion in July and September (~50%) and were virtually absent in the winter. Bacterial and archaeal populations correlated with conductivity and rainfall. High concentrations of dissolved solids, as reflected by high conductivity values (up to 125 mS/cm), occurred in the summer and correlated with high archaeal populations and proportionally lower bacterial populations. Eukaryotes were not detected in January, when total microbial cell numbers were lowest (<105 cells/ml), but eukaryotes increased at low-pH sites (~0.5) during the remainder of the year. This correlated with decreasing water temperatures (50 to 30°C; January to November) and increasing numbers of prokaryotes (108 to 109 cells/ml). T. ferrooxidans was in highest abundance (>30%) at moderate pHs and temperatures (~2.5 and 20°C) in sites that were peripheral to primary acid-generating sites and lowest (0 to 5%) at low-pH sites (pH ~0.5) that were in contact with the ore body. L. ferrooxidans was more widely distributed with respect to geochemical conditions (pH = 0 to 3; 20 to 50°C) but was more abundant at higher temperatures and lower pHs (~40°C; pH ~0.5) than T. ferrooxidans.

* Corresponding author. Mailing address: Department of Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institute, McLean Lab Mailstop #8, Woods Hole, MA 02543. Phone: (508) 289-3620. Fax: (508) 289-2183. E-mail: kedwards{at}whoi.edu.

Applied and Environmental Microbiology, August 1999, p. 3627-3632, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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