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Applied and Environmental Microbiology, August 1999, p. 3705-3709, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Quantification of the Flavonoid-Degrading Bacterium Eubacterium ramulus in Human Fecal Samples with a Species-Specific Oligonucleotide Hybridization Probe

Rainer Simmering,1 Brigitta Kleessen,2 and Michael Blaut1,2,*

Abteilung Gastrointestinale Mikrobiologie, Deutsches Institut für Ernährungsforschung,2 and Institut für Ernährungsforschung, Universität Potsdam,1 14558 Bergholz-Rehbrücke, Germany

Received 5 February 1999/Accepted 25 May 1999

To investigate the occurrence of the flavonoid-degrading bacterium Eubacterium ramulus in the human intestinal tract, an oligonucleotide probe designated S-S-E.ram-0997-a-A-18 was designed and validated, with over 90 bacterial strains representing the dominant described human fecal flora. Application of S-S-E.ram-0997-a-A-18 to fecal samples from 20 subjects indicated the presence of E. ramulus in each individual tested in numbers from 4.4 × 107 to 2.0 × 109 cells/g of fecal dry mass. Six fecal E. ramulus isolates were recognized by S-S-E.ram-0997-a-A-18 but exhibited different band patterns when analyzed by randomly amplified polymorphic DNA.

* Corresponding author. Mailing address: Deutsches Institut für Ernährungsforschung, Abteilung Gastrointestinale Mikrobiologie, and Institut für Ernährungsforschung, Universität Potsdam, Arthur-Scheunert-Allee 114-116, D-14558 Bergholz-Rehbrücke, Germany. Phone: 49-33200-88470. Fax: 49-33200-88407. E-mail: blaut{at}www.dife.de.

Applied and Environmental Microbiology, August 1999, p. 3705-3709, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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