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Applied and Environmental Microbiology, August 1999, p. 3727-3729, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] Assay Is a Fast and Reliable Method for Colorimetric Determination of Fungal Cell Densities

Florian M. Freimoser, Claude A. Jakob,dagger Markus Aebi, and Urs Tuor*

Institut f<A><AC>u</AC><AC>&cjs1168;</AC></A>r Mikrobiologie, Eidgenössische Technische Hochschule, CH-8092 Zürich, Switzerland

Received 15 January 1999/Accepted 8 May 1999

The entomopathogenic fungus Neozygites parvispora (Entomophthorales: Zygomycetes) grows in vitro as irregularly rod-shaped hyphal bodies in a complex medium. In order to simplify the medium composition and determine growth-promoting compounds for the cultivation of this fungus, we were looking for a rapid and quantitative method to estimate the number of living cells in small volumes of liquid culture. A colorimetric method for the determination of cell densities using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] proved to be more accurate and timesaving than conventional hemocytometer counting.

* Corresponding author. Mailing address: ETH Zürich, Institute für Mikrobiologie, Schmelzbergstr. 7, CH-8092 Zürich, Switzerland. Phone: 41/1/632 40 01. Fax: 41/1/632 11 48. E-mail: tuor{at}micro.biol.ethz.ch.

dagger Present address: Department for Anatomy & Cell Biology, McGill University, Montreal, Quebec, Canada H3A 2B2.

Applied and Environmental Microbiology, August 1999, p. 3727-3729, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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