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Applied and Environmental Microbiology, August 1999, p. 3738-3741, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Identification of Ruminococcus
flavefaciens as the Predominant Cellulolytic Bacterial Species of
the Equine Cecum
Veronique
Julliand,1,*
Albane
de
Vaux,1
Liliane
Millet,2 and
Gerard
Fonty2
Laboratoire associé de Recherches Zootechniques
INRA-ENESAD, 21036 Dijon Cedex,1 and
Laboratoire de Microbiologie, INRA, Centre de Recherches de
Clermont-Ferrand-Theix, 63122 Saint-Genès-Champanelle,2 France
Received 8 February 1999/Accepted 10 May 1999
Detection and quantification of cellulolytic bacteria with
oligonucleotide probes showed that Ruminococcus
flavefaciens was the predominant species in the pony and donkey
cecum. Fibrobacter succinogenes and Ruminococcus
albus were present at low levels. Four isolates, morphologically
resembling R. flavefaciens, differed from ruminal strains
by their carbohydrate utilization and their end products of cellobiose fermentation.
*
Corresponding author. Mailing address: Laboratoire
associé de Recherches Zootechniques INRA-ENESAD, BP 1607, 21036 Dijon Cedex, France. Phone: 33.(0)3.80.77.25.59. Fax:
33.(0)3.80.77.25.84. E-mail: v.julliand{at}enesad.fr.
Applied and Environmental Microbiology, August 1999, p. 3738-3741, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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