This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gabler, M.
Right arrow Articles by Fischer, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gabler, M.
Right arrow Articles by Fischer, L.
Agricola
Right arrow Articles by Gabler, M.
Right arrow Articles by Fischer, L.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, August 1999, p. 3750-3753, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Production of a New D-Amino Acid Oxidase from the Fungus Fusarium oxysporum

Matthias Gabler and Lutz Fischer*

Institute of Biochemistry and Biotechnology, Technical University of Braunschweig, 38106 Braunschweig, Germany

Received 16 February 1999/Accepted 25 May 1999

The fungus Fusarium oxysporum produced a D-amino acid oxidase (EC 1.4.3.3) in a medium containing glucose as the carbon and energy source and ammonium sulfate as the nitrogen source. The specific D-amino acid oxidase activity was increased up to 12.5-fold with various D-amino acids or their corresponding derivatives as inducers. The best inducers were D-alanine (2.7 µkat/g of dry biomass) and D-3-aminobutyric acid (2.6 µkat/g of dry biomass). The addition of zinc ions was necessary to permit the induction of peroxisomal D-amino acid oxidase. Bioreactor cultivations were performed on a 50-liter scale, yielding a volumetric D-amino acid oxidase activity of 17 µkat liter-1 with D-alanine as an inducer. Under oxygen limitation, the volumetric activity was increased threefold to 54 µkat liter-1 (3,240 U liter-1).

* Corresponding author. Mailing address: Institute of Biochemistry and Biotechnology, Technical University of Braunschweig, Spielmannstr. 7, 38106 Braunschweig, Germany. Phone: 49-531-3915730. Fax: 49-531-3915763. E-mail: L.Fischer{at}tu-bs.de.

Applied and Environmental Microbiology, August 1999, p. 3750-3753, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.




Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»