This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mendum, T. A.
Right arrow Articles by Hirsch, P. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mendum, T. A.
Right arrow Articles by Hirsch, P. R.
Agricola
Right arrow Articles by Mendum, T. A.
Right arrow Articles by Hirsch, P. R.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, September 1999, p. 4155-4162, Vol. 65, No. 9
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Use of Molecular and Isotopic Techniques To Monitor the Response of Autotrophic Ammonia-Oxidizing Populations of the beta  Subdivision of the Class Proteobacteria in Arable Soils to Nitrogen Fertilizer

T. A. Mendum,1,2,* R. E. Sockett,2 and P. R. Hirsch1

IACR---Rothamsted, Harpenden, Hertfordshire AL5 2JQ,1 and Institute of Genetics, Queens Medical Centre, University of Nottingham, Nottingham NG7 2UH,2 United Kingdom

Received 15 April 1999/Accepted 9 July 1999

This study examined the effects of NH4NO3 fertilizer on the size and activity of nitrifying, autotrophic, ammonia-oxidizing populations of the beta  subdivision of the class Proteobacteria in arable soils. Plots under different long-term fertilizer regimes were sampled before and after NH4NO3 additions, and the rates of nitrification were determined by 15N isotopic pool dilution assays. Ammonia-oxidizing populations in the plots were quantified by competitive PCR assays based on the amoA and ribosomal 16S genes. Prior to fertilizer addition, ammonium concentrations and nitrification rates in the plots were comparatively low; ammonia-oxidizing populations were present at 104 to 105 gene copies g of soil-1. Three days after the application of fertilizer, nitrification rates had risen considerably but the size of the ammonia-oxidizing population was unchanged. Six weeks after fertilizer treatment, ammonium concentrations and nitrification rates had fallen while the ammonia-oxidizing populations in plots receiving fertilizer had increased. The rapidity of the rise in nitrification rates observed after 3 days suggests that it results from phenotypic changes in the ammonia-oxidizing bacterial population. Associated increases in population sizes were only observed after 6 weeks and did not correlate directly with nitrifying activity. Phylogenetic analyses of PCR products from one of the plots revealed a population dominated by Nitrosospira-type organisms, similar to those prevalent in other soils.

* Corresponding author. Mailing address: Soil Science Department, IACR---Rothamsted, Harpenden, Hertfordshire AL5 2JQ, United Kingdom. Phone: 44 (0) 1582 763133. Fax: 44 (0) 1582 760981. E-mail: tom.mendum{at}bbsrc.ac.uk.

Applied and Environmental Microbiology, September 1999, p. 4155-4162, Vol. 65, No. 9
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Chu, H., Morimoto, S., Fujii, T., Yagi, K., Nishimura, S. (2009). Soil Ammonia-Oxidizing Bacterial Communities in Paddy Rice Fields as Affected by Upland Conversion History. Soil Sci. 73: 2026-2031 [Abstract] [Full Text]  
  • Chu, H., Fujii, T., Morimoto, S., Lin, X., Yagi, K., Hu, J., Zhang, J. (2007). Community Structure of Ammonia-Oxidizing Bacteria under Long-Term Application of Mineral Fertilizer and Organic Manure in a Sandy Loam Soil. Appl. Environ. Microbiol. 73: 485-491 [Abstract] [Full Text]  
  • Adviento-Borbe, M. A. A., Doran, J. W., Drijber, R. A., Dobermann, A. (2006). Soil Electrical Conductivity and Water Content Affect Nitrous Oxide and Carbon Dioxide Emissions in Intensively Managed Soils. J. Environ. Qual. 35: 1999-2010 [Abstract] [Full Text]  
  • Urakawa, H., Maki, H., Kawabata, S., Fujiwara, T., Ando, H., Kawai, T., Hiwatari, T., Kohata, K., Watanabe, M. (2006). Abundance and population structure of ammonia-oxidizing bacteria that inhabit canal sediments receiving effluents from municipal wastewater treatment plants.. Appl. Environ. Microbiol. 72: 6845-6850 [Abstract] [Full Text]  
  • Habteselassie, M. Y., Stark, J. M., Miller, B. E., Thacker, S. G., Norton, J. M. (2006). Gross Nitrogen Transformations in an Agricultural Soil after Repeated Dairy-Waste Application. Soil Sci. 70: 1338-1348 [Abstract] [Full Text]  
  • Enwall, K., Philippot, L., Hallin, S. (2005). Activity and Composition of the Denitrifying Bacterial Community Respond Differently to Long-Term Fertilization. Appl. Environ. Microbiol. 71: 8335-8343 [Abstract] [Full Text]  
  • Jordan, F. L., Cantera, J. J. L., Fenn, M. E., Stein, L. Y. (2005). Autotrophic Ammonia-Oxidizing Bacteria Contribute Minimally to Nitrification in a Nitrogen-Impacted Forested Ecosystem. Appl. Environ. Microbiol. 71: 197-206 [Abstract] [Full Text]  
  • Horz, H.-P., Barbrook, A., Field, C. B., Bohannan, B. J. M. (2004). Ammonia-oxidizing bacteria respond to multifactorial global change. Proc. Natl. Acad. Sci. USA 101: 15136-15141 [Abstract] [Full Text]  
  • Okano, Y., Hristova, K. R., Leutenegger, C. M., Jackson, L. E., Denison, R. F., Gebreyesus, B., Lebauer, D., Scow, K. M. (2004). Application of Real-Time PCR To Study Effects of Ammonium on Population Size of Ammonia-Oxidizing Bacteria in Soil. Appl. Environ. Microbiol. 70: 1008-1016 [Abstract] [Full Text]  
  • Avrahami, S., Conrad, R. (2003). Patterns of Community Change among Ammonia Oxidizers in Meadow Soils upon Long-Term Incubation at Different Temperatures. Appl. Environ. Microbiol. 69: 6152-6164 [Abstract] [Full Text]  
  • Avrahami, S., Conrad, R., Braker, G. (2002). Effect of Soil Ammonium Concentration on N2O Release and on the Community Structure of Ammonia Oxidizers and Denitrifiers. Appl. Environ. Microbiol. 68: 5685-5692 [Abstract] [Full Text]  
  • Briones, A. M., Okabe, S., Umemiya, Y., Ramsing, N.-B., Reichardt, W., Okuyama, H. (2002). Influence of Different Cultivars on Populations of Ammonia-Oxidizing Bacteria in the Root Environment of Rice. Appl. Environ. Microbiol. 68: 3067-3075 [Abstract] [Full Text]  
  • Webster, G., Embley, T. M., Prosser, J. I. (2002). Grassland Management Regimens Reduce Small-Scale Heterogeneity and Species Diversity of {beta}-Proteobacterial Ammonia Oxidizer Populations. Appl. Environ. Microbiol. 68: 20-30 [Abstract] [Full Text]  
  • Dionisi, H. M., Layton, A. C., Harms, G., Gregory, I. R., Robinson, K. G., Sayler, G. S. (2002). Quantification of Nitrosomonas oligotropha-Like Ammonia-Oxidizing Bacteria and Nitrospira spp. from Full-Scale Wastewater Treatment Plants by Competitive PCR. Appl. Environ. Microbiol. 68: 245-253 [Abstract] [Full Text]  
  • Oved, T., Shaviv, A., Goldrath, T., Mandelbaum, R. T., Minz, D. (2001). Influence of Effluent Irrigation on Community Composition and Function of Ammonia-Oxidizing Bacteria in Soil. Appl. Environ. Microbiol. 67: 3426-3433 [Abstract] [Full Text]  
  • Hermansson, A., Lindgren, P.-E. (2001). Quantification of Ammonia-Oxidizing Bacteria in Arable Soil by Real-Time PCR. Appl. Environ. Microbiol. 67: 972-976 [Abstract] [Full Text]  
  • Purkhold, U., Pommerening-Röser, A., Juretschko, S., Schmid, M. C., Koops, H.-P., Wagner, M. (2000). Phylogeny of All Recognized Species of Ammonia Oxidizers Based on Comparative 16S rRNA and amoA Sequence Analysis: Implications for Molecular Diversity Surveys. Appl. Environ. Microbiol. 66: 5368-5382 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»