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Applied and Environmental Microbiology, September 1999, p. 4207-4210, Vol. 65, No. 9
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Detection and Identification of Amanitins in the Wood-Rotting Fungi Galerina fasciculata and Galerina helvoliceps

Shinjiro Muraoka,1,2 Nobuko Fukamachi,3 Kiyohisa Mizumoto,3 and Takao Shinozawa1,*

Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University, Kiryu, Gunma 376-8515,1 Department of Biochemistry, School of Pharmaceutical Sciences, Kitasato University, Shirokane, Minato-ku, Tokyo 108-8641,3 and Research and Development Division, Mori & Company, Ltd., Kiryu, Gunma 376-0051,2 Japan

Received 1 March 1999/Accepted 6 June 1999

More than 600 strains of wood-rotting fungi were screened for the detection of amanitins. Three strains of Galerina fasciculata and 18 strains of Galerina helvoliceps contained amanitins. These strains contained mainly alpha - and beta -amanitins in the native fruit bodies, while alpha - and gamma -amanitins were found in liquid-cultured mycelia. Purified amanitins were confirmed by their chromatographic profiles, spectra (UV, Fourier transform infrared, and atmospheric ionization mass), cytotoxicity for mammalian cell lines (3T3 and SiHa), and inhibitory effects on RNA polymerase II. The results revealed that the purified amanitin fractions from these species are identical to authentic amanitins and suggest that these two species must be handled as poisonous mushrooms.

* Corresponding author. Mailing address: Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University, Kiryu, Gunma 376-8515, Japan. Phone: 81-277-30-1431. Fax: 81-277-30-1431. E-mail: shinozawa{at}bce.gunma-u.ac.jp.

Applied and Environmental Microbiology, September 1999, p. 4207-4210, Vol. 65, No. 9
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Enjalbert, F., Cassanas, G., Rapior, S., Renault, C., Chaumont, J.-P. (2004). Amatoxins in wood-rotting Galerina marginata. Mycologia 96: 720-729 [Abstract] [Full Text]  


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