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Applied and Environmental Microbiology, September 1999, p. 4216-4219, Vol. 65, No. 9
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Isolation, Nucleotide Sequence, and Physiological Relevance of the Gene Encoding Triose Phosphate Isomerase from Kluyveromyces lactis

Concetta Compagno,1,* Francesco Boschi,1 Agnese Daleffe,1 Danilo Porro,2 and Bianca Maria Ranzi1

Dipartimento di Fisiologia e Biochimica Generali, Sezione Biochimica Comparata, Università degli Studi di Milano, 20133 Milan,1 and Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca, 20126 Milan,2 Italy

Received 20 January 1999/Accepted 7 June 1999

Lack of triose phosphate isomerase activity (TIM) is of special interest because this enzyme works at an important branch point of glycolytic flux. In this paper, we report the cloning and sequencing of the Kluyveromyces lactis gene encoding TIM. Unlike Saccharomyces cerevisiae Delta TPI1 mutants, the K. lactis mutant strain was found to be able to grow on glucose. Preliminary bioconversion experiments indicated that, like the S. cerevisiae TIM-deficient strain, the K. lactis TIM-deficient strain is able to produce glycerol with high yield.

* Corresponding author. Mailing address: Dipartimento di Fisiologia e Biochimica Generali, Sezione Biochimica Comparata, University of Milan, Via Celoria 26, 20133 Milan, Italy. Phone: 39 2 70644801. Fax: 39 2 70632811. E-mail: compagno{at}imiucca.csi.unimi.it.

Applied and Environmental Microbiology, September 1999, p. 4216-4219, Vol. 65, No. 9
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Overkamp, K. M., Bakker, B. M., Kotter, P., Luttik, M. A. H., van Dijken, J. P., Pronk, J. T. (2002). Metabolic Engineering of Glycerol Production in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 68: 2814-2821 [Abstract] [Full Text]  


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