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Applied and Environmental Microbiology, September 1999, p. 4234-4238, Vol. 65, No. 9
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Clostridium difficile Cell Attachment Is Modified by Environmental Factors

Anne-Judith Waligora, Marie-Claude Barc, Pierre Bourlioux, Anne Collignon, and Tuomo Karjalainen*

Département de Microbiologie, Faculté de Pharmacie, Université de Paris-Sud, 92296 Châtenay-Malabry Cedex, France

Received 6 January 1999/Accepted 28 June 1999

Adherence of Clostridium difficile to Vero cells under anaerobic conditions was increased by a high sodium concentration, calcium-rich medium, an acidic pH, and iron starvation. The level of adhesion of nontoxigenic strains was comparable to that of toxigenic strains. Depending on the bacterial culture conditions, Vero cells could bind to one, two, or three bacterial surface proteins with molecular masses of 70, 50, and 40 kDa.

* Corresponding author. Mailing address: Département de Microbiologie, Faculté de Pharmacie, Université de Paris-Sud, 92296 Châtenay-Malabry Cedex, France. Phone: (33) 1-46 83 55 49. Fax: (33) 1-46 83 58 83. E-mail: tuomo.karjalainen{at}cep.u-psud.fr.

Applied and Environmental Microbiology, September 1999, p. 4234-4238, Vol. 65, No. 9
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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