This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rossi, F.
Right arrow Articles by Dellaglio, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rossi, F.
Right arrow Articles by Dellaglio, F.
Agricola
Right arrow Articles by Rossi, F.
Right arrow Articles by Dellaglio, F.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, September 1999, p. 4241-4244, Vol. 65, No. 9
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Genus- and Species-Specific PCR-Based Detection of Dairy Propionibacteria in Environmental Samples by Using Primers Targeted to the Genes Encoding 16S rRNA

Franca Rossi,1 Sandra Torriani,1,2 and Franco Dellaglio1,*

Dipartimento Scientifico e Tecnologico, Facoltà di Scienze MM. FF. e NN., Università degli Studi di Verona, 37134 Verona,1 and Istituto di Industrie Agrarie, Università degli Studi di Catania, 95123 Catania,2 Italy

Received 10 February 1999/Accepted 1 July 1999

PCR assays with primers targeted to the genes encoding 16S rRNA were developed for detection of dairy propionibacteria. Propionibacterium thoenii specific oligonucleotide PT3 was selected after partial resequencing. Tests allowed the detection of less than 10 cells per reaction from milk and cheese and 102 cells per reaction from forage and soil.

* Corresponding author. Mailing address: Dipartimento Scientifico e Tecnologico, Facoltà di Scienze MM.FF.NN., Università degli Studi di Verona, Strada Le Grazie, Cà Vignal, 1-37134 Verona, Italy. Phone: 39 045 8098917. Fax: 39 045 8098929. E-mail: dellaglio{at}sci.univr.it.

Applied and Environmental Microbiology, September 1999, p. 4241-4244, Vol. 65, No. 9
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Faye, T., Brede, D. A., Langsrud, T., Nes, I. F., Holo, H. (2004). Prevalence of the Genes Encoding Propionicin T1 and Protease-Activated Antimicrobial Peptide and Their Expression in Classical Propionibacteria. Appl. Environ. Microbiol. 70: 2240-2244 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»