This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, Y.-M.
Right arrow Articles by Liu, J.-K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, Y.-M.
Right arrow Articles by Liu, J.-K.
Agricola
Right arrow Articles by Zhang, Y.-M.
Right arrow Articles by Liu, J.-K.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, January 2000, p. 105-112, Vol. 66, No. 1
0099-2240/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Induction of a Futile Embden-Meyerhof-Parnas Pathway in Deinococcus radiodurans by Mn: Possible Role of the Pentose Phosphate Pathway in Cell Survival

Y.-M. Zhang,1 T.-Y. Wong,1 L.-Y. Chen,2 C.-S. Lin,3 and J.-K. Liu2,*

Department of Biological Sciences,2 and Department of Marine Resources,3 National Sun Yat-sen University, Taiwan, Republic of China, and Department of Microbiology and Molecular Cell Sciences, University of Memphis, Memphis, Tennessee 381521

Received 2 July 1999/Accepted 26 October 1999

Statistical models were used to predict the effects of tryptone, glucose, yeast extract (TGY) and Mn on biomass formation of the highly radioresistant bacterium Deinococcus radiodurans. Results suggested that glucose had marginal effect on biomass buildup, but Mn was a significant factor for biomass formation. Mn also facilitated glucose interactions with other nutrient components. These predictions were verified by in vivo and in vitro experiments. TGY-grown cells metabolized glucose solely by the pentose phosphate pathway (PPP). Although only a fraction of glucose from the medium was transported into the cells, glucose was incorporated into the DNA efficiently after cells were exposed to UV light. The presence of glucose also enhanced the radioresistance of the culture. Mn could induce an Embden-Meyerhof-Parnas (EMP) pathway in D. radiodurans. The EMP pathway and the PPP of the Mn-treated cells oxidized glucose simultaneously at a 6:1 ratio. Although glucose was hydrolyzed rapidly by the Mn-treated cells, most glucose was released as CO2. Mn-treated cultures retained less glucose per cell than cells grown without Mn, and still less glucose was incorporated into the DNA after cells were exposed to UV light. Mn-treated cells were also more sensitive to UV light. Results suggested that metabolites of glucose generated from the PPP enhanced the survival of D. radiodurans. Induction of the EMP pathway by Mn may deplete metabolites for DNA repair and may induce oxidative stress for the cell, leading to reduction of radioresistance.

* Corresponding author. Mailing address: Department of Biological Sciences, National Sun Yat-sen University, 70 Lien-Hai Rd., Kaohsiung, Taiwan 804, Republic of China. Phone: 886-7-525-3620. Fax: 886-7-525-3609. E-mail: jkliu{at}mail.nsysu.edu.tw.

Applied and Environmental Microbiology, January 2000, p. 105-112, Vol. 66, No. 1
0099-2240/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Cytryn, E. J., Sangurdekar, D. P., Streeter, J. G., Franck, W. L., Chang, W.-s., Stacey, G., Emerich, D. W., Joshi, T., Xu, D., Sadowsky, M. J. (2007). Transcriptional and Physiological Responses of Bradyrhizobium japonicum to Desiccation-Induced Stress. J. Bacteriol. 189: 6751-6762 [Abstract] [Full Text]  
  • Raymond, A., Shuman, S. (2007). Deinococcus radiodurans RNA ligase exemplifies a novel ligase clade with a distinctive N-terminal module that is important for 5'-PO4 nick sealing and ligase adenylylation but dispensable for phosphodiester formation at an adenylylated nick. Nucleic Acids Res 35: 839-849 [Abstract] [Full Text]  
  • Couee, I., Sulmon, C., Gouesbet, G., El Amrani, A. (2006). Involvement of soluble sugars in reactive oxygen species balance and responses to oxidative stress in plants. J Exp Bot 57: 449-459 [Abstract] [Full Text]  
  • Martins, A., Shuman, S. (2004). An RNA Ligase from Deinococcus radiodurans. J. Biol. Chem. 279: 50654-50661 [Abstract] [Full Text]  
  • Wackett, L. P., Dodge, A. G., Ellis, L. B. M. (2004). Microbial Genomics and the Periodic Table. Appl. Environ. Microbiol. 70: 647-655 [Full Text]  
  • Silver, R. B., Bartman, M. (2002). A Metronome-Like Control of the Calcium Signal Leading to Nuclear Envelope Breakdown and Mitosis in Sand Dollar (Echinaracnius parma) Cells. Biol. Bull. 203: 213-215 [Full Text]  
  • Mrazek, J. (2002). New technology may reveal mechanisms of radiation resistance in Deinococcus radiodurans. Proc. Natl. Acad. Sci. USA 99: 10943-10944 [Full Text]  
  • Karlin, S., Mrázek, J. (2001). Predicted highly expressed and putative alien genes of Deinococcus radiodurans and implications for resistance to ionizing radiation damage. Proc. Natl. Acad. Sci. USA 10.1073/pnas.081077598v1 [Abstract] [Full Text]  
  • Karlin, S., Mrazek, J. (2001). Predicted highly expressed and putative alien genes of Deinococcus radiodurans and implications for resistance to ionizing radiation damage. Proc. Natl. Acad. Sci. USA 98: 5240-5245 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»