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Applied and Environmental Microbiology, January 2000, p. 176-179, Vol. 66, No. 1
0099-2240/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Microcystin Production by Microcystis aeruginosa in a Phosphorus-Limited Chemostat

Hee-Mock Oh,* Seog June Lee, Min-Ho Jang, and Byung-Dae Yoon

Environmental Microbiology Research Unit, Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon 305-600, Korea

Received 13 August 1999/Accepted 29 October 1999

The production of microcystins (MC) from Microcystis aeruginosa UTEX 2388 was investigated in a P-limited continuous culture. MC (MC-LR, MC-RR, and MC-YR) from lyophilized M. aeruginosa were extracted with 5% acetic acid, purified by a Sep-Pak C18 cartridge, and then analyzed by high-performance liquid chromatography with a UV detector and Nucleosil C18 reverse-phase column. The specific growth rate (µ) of M. aeruginosa was within the range of 0.1 to 0.8/day and was a function of the cellular P content under a P limitation. The N/P atomic ratio of steady-state cells in a P-limited medium varied from 24 to 15 with an increasing µ. The MC-LR and MC-RR contents on a dry weight basis were highest at µ of 0.1/day at 339 and 774 µg g-1, respectively, while MC-YR was not detected. The MC content of M. aeruginosa was higher at a lower µ, whereas the MC-producing rate was linearly proportional to µ. The C fixation rate at an ambient irradiance (160 microeinsteins m-2 s-1) increased with µ. The ratios of the MC-producing rate to the C fixation rate were higher at a lower µ. Accordingly, the growth of M. aeruginosa was reduced under a P limitation due to a low C fixation rate, whereas the MC content was higher. Consequently, increases in the MC content per dry weight along with the production of the more toxic form, MC-LR, were observed under more P-limited conditions.

* Corresponding author. Mailing address: Environmental Microbiology Research Unit, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yusong, Taejon 305-600, Korea. Phone: 82-42-860-4321. Fax: 82-42-860-4598. E-mail: heemock{at}mail.kribb.re.kr.

Applied and Environmental Microbiology, January 2000, p. 176-179, Vol. 66, No. 1
0099-2240/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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