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Applied and Environmental Microbiology, January 2000, p. 230-237, Vol. 66, No. 1
0099-2240/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Sources of Escherichia coli in a Coastal Subtropical Environment

Helena M. Solo-Gabriele,1,* Melinda A. Wolfert,1,dagger Timothy R. Desmarais,1 and Carol J. Palmer2

Department of Civil, Architectural and Environmental Engineering, University of Miami, Coral Gables,1 and Nova Southeastern University, Fort Lauderdale,2 Florida

Received 19 February 1999/Accepted 14 October 1999

Sources of Escherichia coli in a coastal waterway located in Ft. Lauderdale, Fla., were evaluated. The study consisted of an extensive program of field measurements designed to capture spatial and temporal variations in E. coli concentrations as well as experiments conducted under laboratory-controlled conditions. E. coli from environmental samples was enumerated by using a defined substrate technology (Colilert-18). Field sampling tasks included sampling the length of the North Fork to identify the river reach contributing high E. coli levels, autosampler experiments at two locations, and spatially intense sampling efforts at hot spots. Laboratory experiments were designed to simulate tidal conditions within the riverbank soils. The results showed that E. coli entered the river in a large pulse during storm conditions. After the storm, E. coli levels returned to baseline levels and varied in a cyclical pattern which correlated with tidal cycles. The highest concentrations were observed during high tide, whereas the lowest were observed at low tide. This peculiar pattern of E. coli concentrations between storm events was caused by the growth of E. coli within riverbank soils which were subsequently washed in during high tide. Laboratory analysis of soil collected from the riverbanks showed increases of several orders of magnitude in soil E. coli concentrations. The ability of E. coli to multiply in the soil was found to be a function of soil moisture content, presumably due to the ability of E. coli to outcompete predators in relatively dry soil. The importance of soil moisture in regulating the multiplication of E. coli was found to be critical in tidally influenced areas due to periodic wetting and drying of soils in contact with water bodies. Given the potential for growth in such systems, E. coli concentrations can be artificially elevated above that expected from fecal impacts alone. Such results challenge the use of E. coli as a suitable indicator of water quality in tidally influenced areas located within tropical and subtropical environments.

* Corresponding author. Mailing address: University of Miami, Department of Civil, Arch., and Environmental Engineering, P.O. Box 248294, Coral Gables, FL 33124-0630. Phone: (305) 284-3489. Fax: (305) 284-3492. E-mail: hmsolo{at}miami.edu.

dagger Present address: Water Resources Division, U.S. Geological Survey, Miami Subdistrict, Miami, FL 33178.

Applied and Environmental Microbiology, January 2000, p. 230-237, Vol. 66, No. 1
0099-2240/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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