This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schwiertz, A.
Right arrow Articles by Blaut, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schwiertz, A.
Right arrow Articles by Blaut, M.
Agricola
Right arrow Articles by Schwiertz, A.
Right arrow Articles by Blaut, M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, January 2000, p. 375-382, Vol. 66, No. 1
0099-2240/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Quantification of Different Eubacterium spp. in Human Fecal Samples with Species-Specific 16S rRNA-Targeted Oligonucleotide Probes

Andreas Schwiertz, Gwenaelle Le Blay, and Michael Blaut*

Deutsches Institut für Ernährungsforschung, Abteilung Gastrointestinale Mikrobiologie, 14558 Bergholz-Rehbrücke, Germany

Received 10 August 1999/Accepted 15 October 1999

Species-specific 16S rRNA-targeted, Cy3 (indocarbocyanine)-labeled oligonucleotide probes were designed and validated to quantify different Eubacterium species in human fecal samples. Probes were directed at Eubacterium barkeri, E. biforme, E. contortum, E. cylindroides (two probes), E. dolichum, E. hadrum, E. lentum, E. limosum, E. moniliforme, and E. ventriosum. The specificity of the probes was tested with the type strains and a range of common intestinal bacteria. With one exception, none of the probes showed cross-hybridization under stringent conditions. The species-specific probes were applied to fecal samples obtained from 12 healthy volunteers. E. biforme, E. cylindroides, E. hadrum, E. lentum, and E. ventriosum could be determined. All other Eubacterium species for which probes had been designed were under the detection limit of 107 cells g (dry weight) of feces-1. The cell counts obtained are essentially in accordance with the literature data, which are based on colony counts. This shows that whole-cell in situ hybridization with species-specific probes is a valuable tool for the enumeration of Eubacterium species in feces.

* Corresponding author. Mailing address: Deutsches Institut für Ernährungsforschung, Abteilung Gastrointestinale Mikrobiologie, Arthur-Scheunert-Allee 114-116, D-14558 Bergholz-Rehbrücke, Germany. Phone: 49-33200-88470. Fax: 49-33200-88407. E-mail: blaut{at}www.dife.de.

Applied and Environmental Microbiology, January 2000, p. 375-382, Vol. 66, No. 1
0099-2240/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Mennink-Kersten, M. A. S. H., Ruegebrink, D., Klont, R. R., Warris, A., Gavini, F., Op den Camp, H. J. M., Verweij, P. E. (2005). Bifidobacterial Lipoglycan as a New Cause for False-Positive Platelia Aspergillus Enzyme-Linked Immunosorbent Assay Reactivity. J. Clin. Microbiol. 43: 3925-3931 [Abstract] [Full Text]  
  • Lay, C., Rigottier-Gois, L., Holmstrom, K., Rajilic, M., Vaughan, E. E., de Vos, W. M., Collins, M. D., Thiel, R., Namsolleck, P., Blaut, M., Dore, J. (2005). Colonic Microbiota Signatures across Five Northern European Countries. Appl. Environ. Microbiol. 71: 4153-4155 [Abstract] [Full Text]  
  • Langlands, S J, Hopkins, M J, Coleman, N, Cummings, J H (2004). Prebiotic carbohydrates modify the mucosa associated microflora of the human large bowel. Gut 53: 1610-1616 [Abstract] [Full Text]  
  • Gueimonde, M., Tolkko, S., Korpimaki, T., Salminen, S. (2004). New Real-Time Quantitative PCR Procedure for Quantification of Bifidobacteria in Human Fecal Samples. Appl. Environ. Microbiol. 70: 4165-4169 [Abstract] [Full Text]  
  • McGarvey, J. A., Miller, W. G., Sanchez, S., Stanker, L. (2004). Identification of Bacterial Populations in Dairy Wastewaters by Use of 16S rRNA Gene Sequences and Other Genetic Markers. Appl. Environ. Microbiol. 70: 4267-4275 [Abstract] [Full Text]  
  • Zoetendal, E. G., Collier, C. T., Koike, S., Mackie, R. I., Gaskins, H. R. (2004). Molecular Ecological Analysis of the Gastrointestinal Microbiota: A Review. J. Nutr. 134: 465-472 [Abstract] [Full Text]  
  • Schoefer, L., Mohan, R., Schwiertz, A., Braune, A., Blaut, M. (2003). Anaerobic Degradation of Flavonoids by Clostridium orbiscindens. Appl. Environ. Microbiol. 69: 5849-5854 [Abstract] [Full Text]  
  • Hold, G. L., Schwiertz, A., Aminov, R. I., Blaut, M., Flint, H. J. (2003). Oligonucleotide Probes That Detect Quantitatively Significant Groups of Butyrate-Producing Bacteria in Human Feces. Appl. Environ. Microbiol. 69: 4320-4324 [Abstract] [Full Text]  
  • Matsuki, T., Watanabe, K., Fujimoto, J., Miyamoto, Y., Takada, T., Matsumoto, K., Oyaizu, H., Tanaka, R. (2002). Development of 16S rRNA-Gene-Targeted Group-Specific Primers for the Detection and Identification of Predominant Bacteria in Human Feces. Appl. Environ. Microbiol. 68: 5445-5451 [Abstract] [Full Text]  
  • Zoetendal, E. G., Ben-Amor, K., Harmsen, H. J. M., Schut, F., Akkermans, A. D. L., de Vos, W. M. (2002). Quantification of Uncultured Ruminococcus obeum-Like Bacteria in Human Fecal Samples by Fluorescent In Situ Hybridization and Flow Cytometry Using 16S rRNA-Targeted Probes. Appl. Environ. Microbiol. 68: 4225-4232 [Abstract] [Full Text]  
  • Harmsen, H. J. M., Raangs, G. C., He, T., Degener, J. E., Welling, G. W. (2002). Extensive Set of 16S rRNA-Based Probes for Detection of Bacteria in Human Feces. Appl. Environ. Microbiol. 68: 2982-2990 [Abstract] [Full Text]  
  • HOPKINS, M.J., MACFARLANE, G.T. (2002). Changes in predominant bacterial populations in human faeces with age and with Clostridium difficile infection. J Med Microbiol 51: 448-454 [Abstract] [Full Text]  
  • Harmsen, H. J. M., Wildeboer-Veloo, A. C. M., Grijpstra, J., Knol, J., Degener, J. E., Welling, G. W. (2000). Development of 16S rRNA-Based Probes for the Coriobacterium Group and the Atopobium Cluster and Their Application for Enumeration of Coriobacteriaceae in Human Feces from Volunteers of Different Age Groups. Appl. Environ. Microbiol. 66: 4523-4527 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»