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Applied and Environmental Microbiology, January 2000, p. 406-412, Vol. 66, No. 1
0099-2240/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Comparison of Animal Infectivity and Nucleic Acid Staining for Assessment of Cryptosporidium parvum Viability in Water

Norman F. Neumann,1 Lyndon L. Gyürek,1,2 Leslie Gammie,3 Gordon R. Finch,2 and Miodrag Belosevic1,4,*

Department of Biological Sciences,1 Department of Civil and Environmental Engineering,2 and Medical Microbiology and Immunology,4 University of Alberta, and EPCOR,3 Edmonton, Alberta T6G 2E9, Canada

Received 16 June 1999/Accepted 16 September 1999

Cryptosporidium parvum oocysts were stained with the fluorogenic dyes SYTO-9 and SYTO-59 and sorted by flow cytometry in order to determine whether the fluorescent staining intensity correlated with the ability of oocysts to infect neonatal CD-1 mice. Oocysts that did not fluoresce or that displayed weak fluorescent intensity when stained with SYTO-9 or SYTO-59 readily established infections in mice, whereas those oocysts that fluoresced brightly did not. Although fluorescent staining profiles varied among different batches of oocysts, a relative cutoff in fluorescent staining intensity that correlated with animal infectivity was observed for all batches.

* Corresponding author. Mailing address: Department of Biological Sciences, CW-405 Biological Sciences Building, University of Alberta, Edmonton, Alberta T6G 2E9, Canada. Phone: (780) 492-1266. Fax: (780) 492-9234. E-mail: mike.belosevic{at}ualberta.ca.

Applied and Environmental Microbiology, January 2000, p. 406-412, Vol. 66, No. 1
0099-2240/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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