This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Moschetti, G.
Right arrow Articles by Coppola, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Moschetti, G.
Right arrow Articles by Coppola, S.
Agricola
Right arrow Articles by Moschetti, G.
Right arrow Articles by Coppola, S.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, January 2000, p. 422-424, Vol. 66, No. 1
0099-2240/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Specific Detection of Leuconostoc mesenteroides subsp. mesenteroides with DNA Primers Identified by Randomly Amplified Polymorphic DNA Analysis

Giancarlo Moschetti, Giuseppe Blaiotta, Francesco Villani, and Salvatore Coppola*

Dipartimento di Scienza degli Alimenti, Sezione di Microbiologia Agraria, Alimentare ed Ambientale e di Igiene, Università degli Studi di Napoli "Federico II," I 80055 Portici, Italy

Received 26 March 1999/Accepted 30 September 1999

Randomly amplified polymorphic DNA analysis using primer 239 (5' CTGAAGCGGA 3') was performed to characterize Leuconostoc sp. strains. All the strains of Leuconostoc mesenteroides subsp. mesenteroides (with the exception of two strains), two strains formerly identified as L. gelidum, and one strain of Leuconostoc showed a common band at about 1.1 kb. This DNA fragment was cloned and sequenced in order to verify its suitability for identifying L. mesenteroides subsp. mesenteroides strains.

* Corresponding author. Mailing address: Dipartimento di Scienza degli Alimenti, Sezione di Microbiologia Agraria, Alimentare ed Ambientale e di Igiene, Università degli Studi di Napoli "Federico II," Via Università 100, I 80055 Portici, Italy. Phone: 0039 081 7755339. Fax: 0039 081 7755339. E-mail: sacoppol{at}cds.unina.it.

Applied and Environmental Microbiology, January 2000, p. 422-424, Vol. 66, No. 1
0099-2240/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Barrangou, R., Yoon, S.-S., Breidt, F. Jr., Fleming, H. P., Klaenhammer, T. R. (2002). Characterization of Six Leuconostoc fallax Bacteriophages Isolated from an Industrial Sauerkraut Fermentation. Appl. Environ. Microbiol. 68: 5452-5458 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»